汞离子适配体亲和柱的制备及在水产品中的应用  

Preparation of mercury ion aptamer affinity column and its application in aquatic products

作　　者：陆明慧 郑文秀 吴世嘉[1] 王周平[1] 段诺[1] LU Minghui;ZHENG Wenxiu;WU Shijia;WANG Zhouping;DUAN Nuo(School of Food Science and Technology,Jiangnan University,Wuxi 214122,China)

机构地区：[1]江南大学食品学院,江苏无锡214122

出　　处：《食品与发酵工业》2025年第5期197-202,共6页Food and Fermentation Industries

基　　金：中国博士后科学基金第11批特别资助项目(2018T110443)。

摘　　要：重金属污染问题已成为我国广泛关注的重大农业生态环境问题,因其污染来源广、残毒时间长,而且具有生物富集及难降解等特点,对农业生态环境、粮食安全和国民健康带来了巨大威胁。该文以汞离子(Hg^(2+))为模式分析物,基于生物素-亲和素特异性结合作用,将Hg^(2+)核酸适配体固定于琼脂糖凝胶上填柱制成Hg^(2+)核酸适配体亲和柱。利用核酸适配体针对Hg^(2+)的高亲和力和高特异性结合,实现对食品中Hg^(2+)的高效富集和脱除。结果显示所制备适配体亲和柱对的最大柱容量为0.025 mg,且对Hg^(2+)表现出良好的特异性,可循环利用12次。可实现对鱼露样品中Hg^(2+)的高效脱除且对其营养成分没有影响。此方法实现稳定高效脱除的同时兼具了造价成本低廉的优点,具有良好的发展前景。Heavy metal pollution has become a major agriculturally environmental concern in China.Because of its wide range of pollution sources,long toxic residues,biological enrichment and difficult to degrade,it poses a great threat to the agricultural ecological environment,food security,and human health.In this work,based on the specific binding of biotin and streptavidin,the mercury ion(Hg^(2+))specific aptamer was modified on the agarose gel to fill the column to make a mercury ion aptamer affinity column.The efficient removal of mercury ions was achieved through high affinity and specificity of aptamer to the target.The maximum column capacity of this aptamer affinity column was 0.025 mg and showed good specificity to mercury ions,and could be reused at least 12 times.The column was further applied to the analysis of Hg^(2+)in fish sauce samples with good removal and no obvious effects on its nutritional components.This method s achieves stable and efficient removal,and has the advantages of low cost and good development prospects.

关 键 词：适配体 亲和柱 重金属 汞离子 水产品 

分 类 号：TS254.7[轻工技术与工程—水产品加工及贮藏工程]