分析化学和生物化学的交叉融合创新实验——核酸分子的荧光检测  

Cross-disciplinary Innovation Experiment:Fluorescence Detection of Nucleic Acid Molecules through Lntegration of Analytical and Biochemical Chemistry

作　　者：杨婵 田硕 郁林 杨茏诚 莫柳婷 林伟英 YANG Chan;TIAN Shuo;YU Lin;YANG Longcheng;MO Liuting;LIN Weiying(College of Chemistry and Chemical Engineering,Guangxi University,Guangxi Nanning 530004,China)

机构地区：[1]广西大学化学化工学院,广西南宁530004

出　　处：《广州化工》2025年第4期190-193,共4页GuangZhou Chemical Industry

摘　　要：以利用荧光分析法进行核酸检测为例,探讨如何将生物化学与分析化学实验交叉融合,形成综合型创新实验,旨在提升本科生的科研素养和实践能力。在生物化学实验室,学生通过凝胶电泳实验验证核酸链置换反应的发生。在分析化学实验室,学生使用紫外可见分光光度计和荧光分光光度计对核酸进行定量。通过整个综合性实验流程,学生不仅能够学习生物化学和分析化学中先进的仪器设备的操作,还能够建立科学研究的思维,激发对科学研究的热爱与信心。Taking the utilization of fluorescence analysis for nucleic acid detection as an example,the integration of Biochemical and Analytical Chemistry experiments to enhance undergraduate students’research literacy and practical skills was explored.In the biochemical laboratory,students validated nucleic acid strand displacement reactions through gel electrophoresis experiments.In the analytical chemistry laboratory,students employed UV-visible spectrophotometry and fluorescence spectrophotometry to quantify nucleic acids.Throughout the comprehensive experimental process,students not only learned the operation of advanced instruments and equipment in Biochemical and Analytical Chemistry,but also developed scientific research thinking,fostering a passion for and confidence in scientific research.

关 键 词：生物化学 分析化学 实验教学 核酸分子 荧光检测 

分 类 号：O69[理学—化学]