基于生物信息学筛选肺腺癌预后相关的关键基因  

Screening of prognostically relevant Hub genes in lung adenocarcinoma based on bioinformatics

作　　者：刘晓东 孙站兵 金慧云 童宇琪 张朝晖 LIU Xiaodong;SUN Zhanbing;JIN Huiyun;TONG Yuqi;ZHANG Zhaohui(School of Public Health,Hengyang Medical School,University of South China,Hengyang 421001,Hunan,China;Hengyang Maternal and Child Health Hospital,Hengyang 421001,Hunan,China)

机构地区：[1]南华大学衡阳医学院公共卫生学院,湖南衡阳421001 [2]衡阳市妇幼保健院,湖南衡阳421001

出　　处：《中南医学科学杂志》2025年第1期12-16,共5页Medical Science Journal of Central South China

基　　金：国家自然科学基金(81573193);湖南省自然科学基金项目(2020JJ4082);湖南省研究生科研创新项目(CX20230995)。

摘　　要：目的基于生物信息学筛选肺腺癌(LUAD)预后相关的关键(Hub)基因。方法通过GEO数据库获得3个LUAD mRNA的芯片数据集(GSE118370、GSE75037和GSE63459)中基于人类样本和匹配的正常肺组织样本,利用GEO2R筛选LUAD和正常组织的差异表达基因(DEG)。采用WebGestalt对DEG进行GO和KEGG分析,通过STRING构建PPI网络并用Cytoscape筛选Hub基因。采用GEPIA结合Kaplan-Meier Plotter分析Hub基因对LUAD患者肿瘤分期、生存的影响。结果3个数据集共筛出2075个DEG,其中353个基因均在3个数据集中存在差异表达。PPI网络筛选出9个Hub基因,其表达水平在LUAD不同分期患者样本间差异无显著性(P>0.05),但其中8个Hub基因(GNG11、ADCY4、PPBP、FPR1、CXCR2、S1PR1、P2RY14、SSTR1)的表达水平与LUAD患者总生存率显著相关。WebGestalt富集分析结果显示,5个Hub基因(PPBP、CXCR2、CXCL2、ADCY4和GNG11)显著富集于趋化因子通路。结论利用生物信息学方法筛选出9个LUAD的Hub基因中有8个(GNG11、ADCY4、PPBP、FPR1、CXCR2、S1PR1、P2RY14、SSTR1)与LUAD预后相关。Aim To screen the prognostic key(Hub)genes in lung adenocarcinoma(LUAD)based on bioinformatics.Methods Human samples and matched normal lung tissue samples from three LUAD mRNA microarray datasets(GSE118370,GSE75037 and GSE63459)were obtained from GEO database,and differentially expressed genes(DEG)between LUAD and normal tissues were identified through GEO2R.The GO and KEGG analysis of DEG were carried out through WebGestalt platform,PPI network was obtained by STRING,and Hub genes were screened by Cytoscape.The effects of Hub genes on tumor staging and survival were analyzed by GEPIA combined with Kaplan-Meier Plotter in LUAD patients.Results There were 2075 DEGs in the three datasets,of which 353 genes were differentially expressed in all three datasets.There was no significant difference in the expression levels of 9 Hub genes screened by PPI network among patients with different stages of LUAD(P>0.05).However,the expression levels of 8 Hub genes(GNG11,ADCY4,PPBP,FPR1,CXCR2,S1PR1,P2RY14,SSTR1)were significantly correlated with overall survival of LUAD patients.WebGestalt re-enrichment analysis showed that 5 Hub genes(PPBP,CXCR2,CXCL2,ADCY4 and GNG11)were significantly enriched in the chemokine pathway.Conclusion By using the bioinformatics method,8 of 9 Hub genes(GNG11,ADCY4,PPBP,FPR1,CXCR2,S1PR1,P2RY14,SSTR1)were found to be associated with LUAD prognosis.

关 键 词：关键基因 肺腺癌 生物信息学 预后 

分 类 号：R734.2[医药卫生—肿瘤]