超声协同中性蛋白酶辅助提取青鱼皮明胶的响应面优化工艺研究  

Optimization of extraction of gelatin from black carp skin assisted by ultrasound and neutral protease by response surface methodology

作　　者：陈文娟[1] 陈建福[2] 庄远红 CHEN Wenjuan;CHEN Jianfu;ZHUANG Yuanhong(Faculty of Food and Health,Zhangzhou City Vocational College,Zhangzhou 363000,Fujian,China;College of Food Engineering,Zhangzhou Institute of Technology,Zhangzhou 363000,Fujian,China;Department of Biological Science and Technology,Minnan Normal University,Zhangzhou 363000,Fujian,China)

机构地区：[1]漳州城市职业学院食品与健康学院,福建漳州363000 [2]漳州职业技术学院食品工程学院,福建漳州363000 [3]闽南师范大学生物科学与技术学院,福建漳州363000

出　　处：《渔业现代化》2025年第1期110-118,共9页Fishery Modernization

基　　金：漳州市自然科学基金(ZZ2024J60);福建省中青年教师教育科研项目(JAT210934)。

摘　　要：为探究青鱼皮明胶的最佳提取工艺,提高青鱼的附加产值。以青鱼皮为原料,以明胶提取率为响应值,基于单因素试验结果,选定关键工艺参数超声时间、酶用量、酶解时间和酶解温度进行Box-Behnken试验设计,探讨各参数及其交互作用对明胶提取率的影响,构建了回归模型,并用红外光谱对所取的明胶进行表征。结果显示:青鱼皮明胶的最佳提取工艺条件为:超声时间125 min、酶用量0.22%、酶解时间154 min和酶解温度45℃,在此条件下进行3次平行试验验证,得到的青鱼皮明胶提取率平均值为18.28%,与预测值之间的相对误差仅为0.22%,这充分证明了所建回归模型的可靠性和高精度。红外光谱表明所提取的物质具有明胶的基本特征。该研究为青鱼皮资源在明胶的开发与应用提供技术参考。The aim is to explore the best extraction technology of gelatin from black carp skin in order to improve the additional output value of black carp.Black carp is one of the four indispensable fish in traditional freshwater culture in China.It is rich in high-quality trace elements such as protein,phosphorus,selenium,iodine,iron and calcium,which are beneficial to human health.However,at present,in the processing of black carp,except fish products,black carp skin as a by-product has not been fully developed and utilized,and its potential economic value has not been fully tapped,resulting in a great waste of resources.Black carp skin is rich in collagen and is an ideal raw material for extracting gelatin.In this work,the effects of ultrasonic time,enzyme dosage,enzymolysis time and enzymolysis temperature on the extraction yield of gelatin from black carp skin were systematically studied by single factor experiment and Box-Behnken experimental design.Gelatin was extracted from black carp skin by ultrasonic treatment and enzymatic hydrolysis.The influence range and trend of each process were preliminarily determined by single factor experiment.Based on the results of single factor experiment,ultrasonic time,enzyme dosage,enzymolysis time and enzymolysis temperature were selected as key process parameters,and Box-Behnken experimental design was carried out.Through regression analysis,a regression model was established to predict the extraction yield of gelatin,and the optimum extraction conditions were determined.The results showed that the optimum extraction conditions of black carp skin gelatin were ultrasonic time 125 min,enzyme dosage 0.22%,enzymolysis time 154 min and enzymolysis temperature 45℃.Under this condition,three parallel experiments were carried out,and the average extraction yield of gelatin from black carp skin was 18.28%,and the relative error between it and the predicted value was only 0.22%,which indicated that the constructed regression model had high reliability and accuracy.In addition,the ext

关 键 词：超声 中性蛋白酶 青鱼皮 明胶 响应面优化工艺 

分 类 号：TS254.4[轻工技术与工程—水产品加工及贮藏工程]