植物乳植杆菌P16缓解DSS诱导的溃疡性结肠炎  

Effect of Lactiplantibacillus plantarum P16 on Relieving DSS-induced Ulcerative Colitis

作　　者：陈智仙 程倩 张彦 CHEN Zhixian;CHENG Qian;ZHANG Yan(National Key Laboratory of Agricultural Microbiology,Yichang 443000,China;The Hubei Provincial Key Laboratory of Yeast Function,Yichang 443000,China;Yi Chang Engineering and Technology Research Center of Nutrition and Health Food,Yichang 443000,China)

机构地区：[1]农业微生物资源发掘与利用全国重点实验室,湖北宜昌443000 [2]酵母功能湖北省重点实验室,湖北宜昌443000 [3]宜昌市营养健康食品工程技术研究中心,湖北宜昌443000

出　　处：《食品工业科技》2025年第6期352-360,共9页Science and Technology of Food Industry

摘　　要：目的:探究植物乳植杆菌P16对葡聚糖硫酸钠(DSS)诱导的小鼠溃疡性结肠炎缓解作用。方法:将BALB/c雄性小鼠随机分为4组(n=10),分别为空白组、模型组、植物乳植杆菌组(P16组)、益生菌对照组(LGG组)。实验设计周期14 d,前7 d正常饮水,后7 d除空白组的其他3组自由饮用3.5%葡聚糖硫酸钠(Dextran sulfate sodium salt,DSS)水溶液,诱导溃疡性结肠炎(Ulcerative colitis,UC)。同时,整个实验过程空白组和模型组灌胃生理盐水0.2 mL/d,P16组和LGG组分别灌胃5×10^(9) CFU/mL对应的益生菌悬液0.2 mL/d。评估疾病活动指数(Disease activity index,DAI),HE染色观察结肠组织病理切片,ELISA检测血清中炎症因子(TNF-α、IL-1β、IL-6、IL-10)。此外,使用16S rRNA系统测序技术检测分析小鼠肠道菌群的变化,研究植物乳植杆菌P16对小鼠结肠炎的影响。结果:与模型组相比,植物乳植杆菌P16极显著降低了结肠炎小鼠的DAI指数(P<0.0001),缓解了小鼠结肠长度萎缩,减轻了DSS对小鼠结肠组织的损伤;同时,植物乳植杆菌P16有效调节了小鼠细胞炎症因子TNF-α、IL-6、IL-1β水平(P<0.01);此外,植物乳植杆菌P16改善了肠道中微生物群落α多样性;并且,经植物乳植杆菌P16干预,调节了小鼠肠道菌群厚壁菌门和拟杆菌门比率,使得Akkermansia的相对丰度增加,Helicobacter、Colidextribacter的相对丰度减少,改善了肠道菌群平衡。结论:植物乳植杆菌P16具有潜在缓解小鼠溃疡性结肠炎的作用,能够有效调节溃疡性结肠炎小鼠的肠道菌群平衡。Objective:To investigate the therapeutic effect of Lactiplantibacillus plantarum P16 on dextran sulfate sodium(DSS)-induced ulcerative colitis in mice.Methods:BALB/c male mice were randomly divided into 4 groups(n=10):control group,model group,L.plantarum P16 group,and LGG group.The experiment lasted for 14 d,with the first 7 d of normal water intake followed by 7 d of free access to 3.5%DSS solution for the model,P16,and LGG to induce ulcerative colitis(UC).Throughout the experiment,the control and model groups were orally gavaged with 0.2 mL/d of saline,while the P16 and LGG groups were orally gavaged with 0.2 mL/d of the corresponding probiotic suspension at a concentration of 5×10^(9) CFU/mL.Disease activity index(DAI)was evaluated,colon tissue pathology was observed by HE staining,and inflammatory factors(TNF-α,IL-1β,IL-6,IL-10)in serum were measured by ELISA.Additionally,16S rRNA sequencing was used to analyze changes in the intestinal microbiota of mice and study the effects of P16 on colitis.Results:Compared with the control group,L.plantarum P16 reduced the DAI index in colitis mice significantly(P<0.0001),alleviated colon length shrinkage,and reduced DSS-induced damage to colon tissues.L.plantarum P16 effectively regulated inflammatory factors TNF-α,IL-6,and IL-1βin mice(P<0.01).Furthermore,L.plantarum P16 improved the alpha diversity of the intestinal microbial community in mice.L.plantarum P16 intervention adjusted the ratio of Firmicutes and Bacteroidetes in the intestinal microbiota of mice,increased the relative abundance of Akkermansia,and decreased the relative abundance of Helicobacter and Colidextribacter,thus improving the balance of the gut microbiota.Conclusion:L.plantarum P16 has the potential to alleviate ulcerative colitis in mice and effectively regulate the intestinal microbiota balance in colitis mice.

关 键 词：植物乳植杆菌P16 结肠炎 肠道菌群 丰度 

分 类 号：TS201.3[轻工技术与工程—食品科学]