沙棘果渣膳食纤维对丙烯酰胺的吸附特性  

Adsorption Characteristics of Dietary Fibres from Sea Buckthorn Pomace on Acrylamide

作　　者：崔蔚然 吕孟玲 白红雨 孙悦 王慧[1] 邵美丽[1] CUI Weiran;LÜMengling;BAI Hongyu;SUN Yue;WANG Hui;SHAO Meili(College of Food Science,Northeast Agricultural Univercity,Harbin 150000,China)

机构地区：[1]东北农业大学食品学院,黑龙江哈尔滨150000

出　　处：《食品工业科技》2025年第6期361-368,共8页Science and Technology of Food Industry

基　　金：国家自然科学基金资助项目(31801518)。

摘　　要：本文旨在探究沙棘果渣膳食纤维(Dietary fiber,DF)吸附丙烯酰胺(Acrylamide,AA)的影响因素和吸附稳定性,同时利用等温吸附模型和动力学模型对沙棘果渣DF吸附AA过程进行拟合。结果表明,随着沙棘果渣DF添加量增加、吸附时间延长,吸附率不断增大;随着AA浓度、体系温度、体系pH增加,吸附率呈先增加后降低的趋势。当沙棘果渣DF添加量8 mg/mL、AA 4μg/mL、pH5、温度35℃、作用时间8 h时,吸附率最大,此时吸附量为44.8μg/g。且沙棘果渣DF-AA复合物具有较好的体内外稳定性。在胃环境中,当pH1.5~2.5、消化时间1~2 h时,沙棘果渣DF-AA复合物比较稳定,AA释放率为0~12.28%;在肠环境中,胆盐浓度0.1%,消化时间3 h时,沙棘果渣DF-AA复合物稳定性最好,AA释放率为14.75%。另外,Langmuir(R^(2)=0.93)、Freundlich(R^(2)=0.90)2种等温吸附模型及拟二级动力学模型(R^(2)=0.94)均能够很好地拟合沙棘果渣DF吸附AA过程,表明该吸附过程既存在物理吸附,又存在化学吸附。本研究可为沙棘DF在AA吸附脱毒方面的应用奠定基础。To explore the influencing factors and adsorption stability of seabuckthorn pomace dietary fiber in adsorbing acrylamide(AA),as well as to fit the adsorption process using isothermal adsorption model and kinetic model.Results indicated that the adsorption rate increased with the addition of seabuckthorn pomace dietary fiber and the extension of adsorption time.However,as the concentration of AA,system temperature,and system pH increased,the adsorption rate initially increased and then decreased.When the seabuckthorn pomace dietary fiber addition was 8 mg/mL,AA concentration was 4μg/mL,pH was 5,temperature was 35℃,and adsorption time was 8 h,the adsorption rate was at its maximum,the adsorbed amount was 44.8μg/g.Moreover,the seabuckthorn pomace dietary fiber-AA complex exhibited good stability both in vitro and in vivo.In the gastric environment,with a pH of 1.5~2.5 and a digestion time of 1~2 h,the seabuckthorn pomace dietary fiber-AA complex remained relatively stable,shen mewith an AA release rate of 0~12.28%.In the intestinal environment,with a bile salt concentration of 0.1%and a digestion time of 3 h,the seabuckthorn pomace dietary fiber-AA complex showed the best stability,with an AA release rate of 14.75%.Additionally,both the Langmuir(R^(2)=0.93)and Freundlich(R^(2)=0.90)isothermal adsorption models,as well as the pseudo-second-order kinetic model(R^(2)=0.94),fitted the adsorption process of AA by seabuckthorn pomace dietary fiber well,indicating the presence of both physical and chemical adsorption.This study lays the foundation for the application of seabuckthorn pomace dietary fiber in AA adsorption detoxification.

关 键 词：沙棘果渣膳食纤维 丙烯酰胺 吸附 体外模拟消化 等温吸附模型 吸附动力学模型 

分 类 号：TS201.1[轻工技术与工程—食品科学]