薏苡仁对葡聚糖硫酸钠诱导的溃疡性结肠炎小鼠肠道菌群的影响  

Effects of Coix Seed on Intestinal Flora Dextran Sulfate Sodiuminduced Ulcerative Colitis in Mice

作　　者：陈孜旋 佟苗苗[1] 吴庆林 孙佳婷 尹小溪 吕添熠 张睦清 李丽 CHEN Zixuan;TONG Miaomiao;WU Qinglin;SUN Jiating;YIN Xiaoxi;LÜTianyi;ZHANG Muqing;LI Li(College of Pharmacy,Hebei Medical University,Shijiazhuang 050017,China;The First Affiliated Hospital of Hebei University of Chinese Medicine,Shijiazhuang 050011,China)

机构地区：[1]河北医科大学药学院,河北石家庄050017 [2]河北中医药大学第一附属医院,河北石家庄050011

出　　处：《食品工业科技》2025年第6期369-376,共8页Science and Technology of Food Industry

基　　金：国家自然科学基金(82003913);河北省高等学校科学技术研究项目(BJK2024082);河北省自然科学基金项目(H2024206502);河北省中医药管理局科研计划项目(2021120)。

摘　　要：目的:研究薏苡仁对葡聚糖硫酸钠诱导的溃疡性结肠炎小鼠结肠炎症及肠道菌群的影响,探究其改善作用机制。方法:小鼠自由饮用3.5%葡聚糖硫酸钠(Dextran sulfate sodium,DSS)溶液7 d制备溃疡性结肠炎模型,同时给予小鼠灌胃薏苡仁水提物进行治疗,试验期间记录小鼠的体重变化、疾病活动指数(Disease activity index,DAI)评分、小鼠结肠长度。通过染色观察小鼠结肠组织病理学变化,检测小鼠结肠内炎症因子的水平,16S rDNA测序技术分析小鼠粪便肠道菌群变化,并通过Pearson系数对关键差异菌的相对丰度及炎症因子水平进行关联性分析。结果:与模型组比较,薏苡仁可以显著改善溃疡性结肠炎小鼠体重减轻,DAI评分升高,结肠缩短(其中CE组结肠平均长度恢复至7.27 cm),肠屏障受损及炎症因子水平升高等病理状态(P<0.05),有效调节结肠炎小鼠肠道菌群多样性变化。在门水平上,薏苡仁水提物可降低结肠炎小鼠变形菌门(Proteobacteria)相对丰度,增加拟杆菌门(Bacteroidetesa)相对丰度;在属水平上,薏苡仁水提物可以增加嗜黏蛋白阿克曼菌属(Akkermansia)和乳杆菌属(Lactobacillus)等与肠屏障修复相关菌属的相对丰度。进一步关联性分析发现薏苡仁调节的关键菌的相对丰度与炎症因子的水平高度关联。结论:薏苡仁可通过调节溃疡性结肠炎小鼠肠道菌群,降低炎症因子水平,修复结肠粘膜屏障,进而缓解结肠炎症状。Objective:To investigate the effect of coix seed on colitis and intestinal flora in mice induced by dextran sulfate sodium(DSS)and explore its mechanism of action.Methods:UC was induced in mice by freely drinking 3.5%DSS solution for 7 d.The coix seed extractions(CE)were given to the mice by gavage for intervention.The changes in body weight,disease activity index(DAI)scores,and colon length of mice were recorded.Colonic histopathological changes were observed by staining,levels of inflammatory factors in the colon of mice were detected,and 16S rDNA sequencing technology was used to analyze changes in fecal intestinal flora of mice.Pearson correlation coefficient was used to analyze the correlation between the relative abundance of key differential bacteria and the levels of inflammatory factors.Results:Compared with the model group,CE significantly improved weight loss,prevented the increase of DAI scores,restored the colon length(the colon length of CE group restored to 7.27 cm),decreased intestinal barrier damage and levels of inflammatory factors in mice(P<0.05),CE could also effectively regulate the changes in intestinal flora diversity in mice with UC.At the phylum level,CE reduced the relative abundance of Proteobacteria and increased the relative abundance of Bacteroidetes in colitis mice.At the genus level,CE increased the relative abundance of Akkermansia and Lactobacillus,which were associated with intestinal barrier repair.Further correlation analysis revealed a strong association between the relative abundance of key bacteria regulated by CE and the levels of inflammatory factors.Conclusion:Coix seed can alleviate colitis symptoms of mice in UC by regulating the intestinal flora,reducing levels of inflammatory factors,repairing colonic mucosal barrier,and thus relieving colonic inflammation.

关 键 词：薏苡仁 溃疡性结肠炎 炎症因子 肠道菌群 关联性分析 

分 类 号：R932[医药卫生—生药学]