Evaluation of chicken chorioallantoic membrane model for tumor imaging and drug development:Promising findings  

作　　者：Lizhen Wang Junjie Yan XinyuWang Yuping Xu Donghui Pan Chongyang Chen Ying Shao Xiangjun Song Kezong Qi Min Yang Jian Tu 

机构地区：[1]Anhui Province Engineering Laboratory for Animal Food Quality and Bio-safety,College of Animal Science and Technology,Anhui Agricultural University,Hefei,China [2]NHC Key Laboratory of Nuclear Medicine,Jiangsu Key Laboratory of Molecular Nuclear Medicine,Jiangsu Institute of Nuclear Medicine,Wuxi,China

出　　处：《Animal Models and Experimental Medicine》2025年第2期287-294,共8页动物模型与实验医学(英文)

基　　金：This study was supported by the National Natural Science Foundation of China(grant numbers:31972644,32272959);the University Synergy Innovation Program of Anhui Province(grant number:GXXT-2019-035);the Jiangsu Provincial Medical Innovation Team(grant number:CXTDA2017024);the leading technology foundation research project of Jiangsu Province(grant number:BK20192005).

摘　　要：Background:The chicken chorioallantoic membrane(CAM)model is a potential alter-native to the mouse model based on the 3R principles.However,its value for deter-mination of the in vivo behaviors of radiolabeled peptides through positron emission tomography(PET)imaging needed investigation.Herein,the chicken CAM tumor models were established,and their feasibility was evaluated for evaluating the imag-ing properties of radiolabeled peptides using a 68Ga-labeled HER2 affibody.Methods:Two human breast cancer cell lines were inoculated into chicken CAM and mice,respectively.The tumor-targeting potential and pharmacokinetic profile of a 68Ga-labeled affibody,68Ga-MZHER,in both tumor models were also determined.Results:The tumor-formation time in chicken CAM model was shorter than that of mouse model.The uptake values of human epithelial growth factor receptor-2(HER2)-positive Bcap37 tumors in chicken CAM and mouse models were 5.36±0.26%ID/g and 5.26±0.43%ID/g at 30 min postinjection of 68Ga-MZHER,respectively.At the same time points,the uptake values of HER2-negative MDA-MB-231 tumors in the chicken CAM models and mouse models were 1.57±0.15%ID/g and 1.67±0.25%ID/g,respectively.Ex vivo biodistribution confirmed that more radioactivity accu-mulated in Bcap37 tumors than in MDA-MD-231 tumors in both CAM and mouse models.Conclusion:In this study,the CAM tumor model was successfully prepared.The chicken CAM model is a novel tool for quickly determining the in vivo properties of radiolabeled peptides targeting biomarkers.It may be beneficial for early monitoring of the therapeutic effect of a new drug through PET imaging with specific peptides.

关 键 词：68Ga AFFIBODY chicken embryo CAM HER2 PET imaging 

分 类 号：R73-3[医药卫生—肿瘤]