Effect of macrophage- to- myofibroblast transition on silicosis  

作　　者：Fei Geng Jingrou Xu Xichen Ren Ying Zhao Yuhao Cai Yaqian Li Fuyu Jin Tian Li Xuemin Gao Wenchen Cai Hong Xu Zhongqiu Wei Na Mao Ying Sun Fang Yang 

机构地区：[1]Hebei Key Laboratory for Organ Fibrosis Research,School of Public Health,North China University of Science and Technology,Tangshan,China [2]Department of Pathology,Hebei Key Laboratory for Chronic Diseases,School of Basic Medical Sciences,North China University of Science and Technology,Tangshan,China

出　　处：《Animal Models and Experimental Medicine》2025年第2期363-371,共9页动物模型与实验医学(英文)

基　　金：The National Natural Science Foundation of China(no.82204006);the Science and Technology of Project of Hebei Education Department(QN2022009);the Provincial Graduate Student Innovation Funding Project of Hebei Province(CXZZBS2022104);the National Natural Science Foundation of Hebei Province(H2020209292).

摘　　要：Background:The aim was to explore the effect of macrophage polarization and macrophage-to-myofibroblast transition(MMT)in silicosis.Methods:Male Wistar rats were divided into a control group and a silicosis group developed using a HOPE MED 8050 dynamic automatic dusting system.Murine mac-rophage MH-S cells were randomly divided into a control group and an SiO_(2) group.The pathological changes in lung tissue were observed using hematoxylin and eosin(HE)and Van Gieson(VG)staining.The distribution and location of macrophage marker(F4/80),M1 macrophage marker(iNOS),M2 macrophage marker(CD206),and myofibroblast marker(α-smooth muscle actin[α-SMA])were detected using immu-nohistochemical and immunofluorescent staining.The expression changes in iNOS,Arg,α-SMA,vimentin,and type I collagen(Col I)were measured using Western blot.Results:The results of HE and VG staining showed obvious silicon nodule formation and the distribution of thick collagen fibers in the lung tissue of the silicosis group.Macrophage marker F4/80 increased gradually from 8 to 32 weeks after exposure to silica.Immunohistochemical and immunofluorescent staining results revealed that there were more iNOS-positive cells and some CD206-positive cells in the lung tissue of the silicosis group at 8 weeks.More CD206-positive cells were found in the silicon nodules of the lung tissues in the silicosis group at 32 weeks.Western blot analysis showed that the expressions of Inducible nitric oxide synthase and Arg protein in the lung tissues of the silicosis group were upregulated compared with those of the con-trol group.The results of immunofluorescence staining showed the co-expression of F4/80,α-SMA,and Col I,and CD206 andα-SMA were co-expressed in the lung tissue of the silicosis group.The extracted rat alveolar lavage fluid revealed F4/80+α-SMA+,CD206+α-SMA+,and F4/80+α-SMA+Col I+cells using immunofluorescence staining.Similar results were also found in MH-S cells induced by SiO_(2).Conclusions:The development of silicosis is accompanied by macroph

关 键 词：MACROPHAGE macrophage-to-myofibroblast transition SILICOSIS 

分 类 号：R13[医药卫生—劳动卫生]