Dynamic landscape of a competing endogenous RNA network in early-onset preeclampsia under hypoxia conditions  

作　　者：Chun-Yan Wei Xiao-Lin Hu 

机构地区：[1]Department of Gynecology,Hospital of Obstetrics and Gynecology,Fudan University,Shanghai 200011,China [2]Laboratory for Reproductive Immunology,Hospital of Obstetrics and Gynecology,Fudan University,Shanghai 200011,China [3]School of Public Health,Shanghai Jiao Tong University School of Medicine,Shanghai 200011,China.

出　　处：《Reproductive and Developmental Medicine》2024年第4期233-241,共9页生殖与发育医学(英文版)

基　　金：National Natural Science Foundation of China(No.82303948)。

摘　　要：Objective:Early-onset preeclampsia(EOPE)manifests as elevated blood pressure and indications of organ damage,predominantly in the kidneys,in women before 34 weeks of gestation.A growing body of evidence suggests that hypoxia triggers a series of maladaptive responses culminating in the progression of EOPE.However,the precise mechanisms underlying these processes remain largely undefined.This study aimed to map the dynamic interplay within the competing endogenous RNA(ceRNA)network in EOPE under hypoxic conditions to provide insights into its molecular etiology.Methods:To investigate the oxidative stress response in EOPE,RNA sequencing data(ID:GSE119265)were retrieved from the Gene Expression Omnibus database for exhaustive analysis.Oxygen-sensitive differentially expressed genes(DEGs)were identified by comparing EOPE samples to controls at 5%and 20%O2 concentrations,respectively.Furthermore,the STRING database facilitated the construction of a protein–protein interaction network,highlighting critical hub genes among the identified DEGs.Results:An intricate ceRNA network encompassing four representative DEGs(AOC1,DCN,TGFB1,andSYNE3)and seven differentially expressed long non-coding RNAs(HCG27,MEG3,XIST,LINC01119,LINC00964,LINC01118,andLINC01588)was established.Conclusions:Our analysis revealed a set of long non-coding RNA that respond to hypoxic stress,shedding light on their possible roles in the oxygen-dependent pathophysiology of EOPE.These insights offer new avenues for targeted EOPE diagnosis and treatment.Further studies are required to elucidate their precise functions.

关 键 词：Early-onset preeclampsia HYPOXIA RNA-SEQ Competing endogenous RNA 

分 类 号：R714.244[医药卫生—妇产科学]