葡萄糖流加对多黏菌素E生物合成代谢的影响  

The Effect of Glucose Fed-batch on the Biosynthesis and Metabolism of Polymyxin E

作　　者：刘丹 LIU Dan(Hebei Shengxue Dacheng Tangshan Pharmaceutical Co.,Ltd,Tangshan 064000,China)

机构地区：[1]河北圣雪大成唐山制药有限责任公司,河北唐山064000

出　　处：《工业微生物》2025年第1期185-187,共3页Industrial Microbiology

摘　　要：文章为考察种子培养过程中葡萄糖流加对多黏菌素E生物合成代谢的影响,对发酵过程中的DO、CER、OUR、RQ和活菌细胞量等重要生理参数实施了在线检测,并对黏菌素效价E2和E1进行了分析。研究显示,种子发酵过程中流加葡萄糖过多,与CER和E2/E1均呈正相关,碳阻遏效应显著;通过流加液体葡萄糖,可使糖点在0.03~0.06 g/100 m L,CER为44.1 mmol/(L·h);当菌体处于最佳产素期,且RQ在1.0~1.2时,移种进入发酵培养,效价涨幅较高,发酵培养黏菌素效价可提高到75万U/mL,提升7.14%。In order to investigate the effect of glucose fed-batch during seed cultivation on the biosynthesis and metabolism of polymyxin E,the paper conducted online detection on important physiological parameters such as DO,CER,OUR,RQ,and viable cell count during fermentation,and analyzed the potency of polymyxin E2 and E1.Excessive addition of glucose during seed fermentation is positively correlated with CER and E2/E1,indicating a significant carbon catabolite repression.By adding liquid glucose,the sugar content was adjusted to 0.03~0.06 g/100 mL,with a CER of 44.1 mmol/(L·h).The bacterial cells were in the optimal period of protein production,and when the RQ was between 1.0~1.2,they were transferred to the fermentation culture,resulting in a significant increase in potency.The colistin potency in the fermentation culture increased to 750000 U/mL,with an increase of 7.14%.

关 键 词：多黏菌素E 发酵 呼吸商 代谢特性 组分 

分 类 号：TQ927[轻工技术与工程—发酵工程]