Shenmai Injection Reduces Cardiomyocyte Apoptosis Induced by Doxorubicin through miR-30a/Bcl-2  

作　　者：ZHANG Xiao-nan LI Yan-yang LYU Shi-chao JIA Qiu-jin ZHANG Jun-ping LIU Long-tao 

机构地区：[1]Department of Cardiovascular Medicine,National Clinical Research Center for Chinese Medicine Cardiology,Xiyuan Hospital,China Academy of Chinese Medical Sciences,Beijing,100091,China [2]Department of Integrated Traditional Chinese and Western Medicine,Tianjin Medical University Cancer Institute and Hospital,Tianjin,300060,China [3]Department of Cardiovascular Medicine,First Teaching Hospital of Tianjin University of Traditional Chinese Medicine,Tianjin,300193,China [4]Department of Cardiovascular Medicine,Tianjin Key Laboratory of Traditional Research of Traditional Chinese Medicine Prescription and Syndrome,Tianjin,300193,China

出　　处：《Chinese Journal of Integrative Medicine》2025年第3期240-250,共11页中国结合医学杂志(英文版)

基　　金：Supported by the National Natural Science Foundation of China(No.81904118);Hospital Capability Enhancement Project of Xiyuan Hospital,China Academy of Chinese Medical Sciences(No.XYZX0201-09);the Special Programme for the Cultivation of Outstanding Young Scientific and Technological Talents of the China Academy of Chinese Medical Sciences(No.ZZ17-YQ-005)。

摘　　要：Objective:To explore the molecular mechanism of Shenmai Injection(SMI)against doxorubicin(DOX)induced cardiomyocyte apoptosis.Methods:A total of 40 specific pathogen-free(SPF)male Sprague Dawley(SD)male rats were divided into 5 groups based on the random number table,including the control group,the model group,miR-30a agomir group,SMI low-dose(SMI-L)group,and SMI high-dose(SMI-H)group,with 8 rats in each group.Except for the control group,the rats were injected weekly with DOX(2 mg/kg)in the tail vein for 4 weeks to induce myocardial injury,and were given different regimens of continuous intervention for 2 weeks.Cardiac function was detected by echocardiography and myocardial pathological changes were observed by Van Gieson(VG)staining.Myocardial injury serum markers,including creatine kinase(CK),lactate dehydrogenase(LDH),troponin T(c Tn T),N-terminal pro-brain natriuretic peptide(NT-pro BNP),soluble ST2(sST2),and growth differentiation factor-15(GDF-15)were detected by enzyme linked immunosorbent assay(ELISA).Cardiomyocyte apoptosis was observed by terminal deoxynucleotidyl transferase-mediated biotinylated d UTP triphosphate nick end labeling(TUNEL)and transmission electron microscopy,and the expressions of target proteins and m RNA were detected by Western blot and quantitative real time polymerase chain reaction(qRT-RCR),respectively.Results:The treatment with different doses of SMI reduced rat heart mass index and left ventricular mass index(P<0.05),significantly improved the left ventricular ejection fraction(P<0.05),decreased the levels of serum CK,LDH,cTnT,and NT-proBNP(P<0.05 or P<0.01),reduced the levels of serum sST2 and GDF-15(P<0.05 or P<0.01),decreased the collagen volume fraction,reduced the expressions of rat myocardial typeⅠand typeⅢcollagen(P<0.05 or P<0.01),and effectively alleviated myocardial fibrosis.And the study found that SMI promoted the expression levels of miR-30a and Bcl-2 in myocardium,and down-regulated the expression of Bax,which inhibited the activation of Caspase-3 and Caspa

关 键 词：Shenmai Injection DOXORUBICIN CARDIOTOXICITY miR-30a/Bcl-2 APOPTOSIS 

分 类 号：R285[医药卫生—中药学]