油莎豆块茎特异性表达基因鉴定及分析  

Identification and Analysis of Tuber-specific Expression Genes in Cyperus esculentus

作　　者：张向歌 陈晨 程珊 李春鑫[1] 朱雅婧 许欣然[1] 王会伟[1] Xiangge Zhang;Chen Chen;Shan Cheng;Chunxin Li;Yajing Zhu;Xinran Xu;Huiwei Wang(Industrial Crop Research Institute,Henan Academy of Agricultural Sciences,Zhengzhou 450002,China)

机构地区：[1]河南省农业科学院经济作物研究所,郑州450002

出　　处：《植物学报》2025年第1期33-48,共16页Chinese Bulletin of Botany

基　　金：河南省重大科技专项(No.211100110100);河南省重点研发与推广专项(科技攻关)(No.242102110305,No.242102110290)。

摘　　要：块茎是油莎豆(Cyperusesculentus)独特且极其重要的器官,富含油脂且具有与种子类似的繁殖能力,研究油莎豆块茎特异性表达基因对于解析其块茎特异性生长发育(尤其是油脂积累)的调控机理具有重要意义。通过对油莎豆主要器官(根、叶、分蘖节、匍匐茎和块茎)进行转录组测序,全面筛选块茎特异性表达基因,并分析相关基因的功能。结果表明,分别以根、叶、分蘖节和匍匐茎为对照,经过多组比对分析后共鉴定出155个块茎特异性表达基因;GO富集分析显示,与种子发育、种子油体合成、油脂储存、脱落酸响应、非生物刺激响应和蛋白折叠相关的7个GO条目显著富集,这些GO条目涉及的基因恰好反映出油莎豆块茎独特的类似种子的发育特性。其中,CESC_00080和CESC_16572编码油体钙蛋白,CESC_08636、CESC_12549和CESC_17828编码油体蛋白,均参与植物油体的形成,而油体形成是植物完成油脂储存的关键步骤,表明这些油体形成相关基因在块茎中的特异性表达可能是油莎豆在块茎中储存大量油脂的关键所在。此外,还筛选出8个块茎特异性表达转录因子编码基因,如CESC_00448(编码abscisicacidinsensitive5-likeproteinABI5)和CESC_03736(编码heat stress transcription factor C),而在鉴定到的块茎特异性表达基因中发现一些潜在的靶基因,表明这些转录因子可能调控各自靶基因的特异性表达。综上,研究结果可为油莎豆块茎发育相关基因调控网络的构建以及块茎特异性基因表达的分子机制解析提供重要参考。Tuber is a unique and extremely important organ of Cyperus esculentus,which is rich in oil and has the re-production ability similar to seed.It is of great significance to study the specific expression genes in the tubers of C.es-culentus for analyzing the regulation mechanism of tuber-specific growth and development(especially oil accumulation).Through transcriptome sequencing of the main organs(root,leaf,tillering node,stolon and tuber)of C.esculentus,the genes specifically expressed in tubers were comprehensively screened and the functions of related genes were analyzed.The results showed that a total of 155 tuber-specific expression genes were identified after multiple sets of comparative analysis by taking root,leaf,tillering node and stolon as reference,respectively.GO enrichment analysis showed that 7 GO terms including seed development,seed oilbody biogenesis,oil storage,abscisic acid response,response to abiotic stimulus and protein folding were significantly enriched,and some of the genes involved in these GO terms just reflected the unique development characteristics similar to seed of C.esculentus tubers.Among them,CESC_00080 and CESC_16572 encode caleosin,and meanwhile,CESC_08636,CESC_12549 and CESC_17828 encode oleosin,all of which are involved in the formation of plant oil bodies.Since oil body formation is a key step for plants to complete oil storage,it is indicated that the specific expression of these oil body formation-related genes in tubers may be the key to the storage of large amounts of oil in the tubers of C.esculentus.In addition,this study also screened eight tuber-specific expression transcription factor genes,such as CESC_00448(abscisic acid insensitive 5-like protein ABI5)and CESC_03736(heat stress transcription factor C),some of whose potential target genes were found in identified tu-ber-specific expression genes,indicating that these transcription factor genes may regulate the specific expression of their respective target genes.In summary,the results of this study can provide an

关 键 词：油莎豆 块茎 转录组测序 特异性表达 

分 类 号：S565.9[农业科学—作物学]