PPAN modulates mouse male germ cell development via maintaining nucleolar homeostasis  

作　　者：Yu Tian Yufan Wang Qing Tian Guiping Cheng Li-quan Zhou 

机构地区：[1]Institute of Reproductive Health,Tongji Medical College,Huazhong University of Science and Technology,Wuhan,Hubei 430030,China [2]Department of Gynecology and Obstetrics,Zhongnan Hospital of Wuhan University,Wuhan,Hubei 430030,China

出　　处：《Genes & Diseases》2025年第1期25-28,共4页基因与疾病(英文)

基　　金：supported by the National Natural Science Foundation of China(No.NSFC 32170820);the National Key R&D Program of China(No.2022YFC2702704);the Program for HUST Academic Frontier Youth Team.

摘　　要：In mammalian cells,the nucleolus serves as a factory for organizing ribosomal RNA(rRNA)synthesis and ribosomal biogenesis.Moreover,by interacting with rRNA genes and heterochromatin,nucleolus also functions in chromatin organization and reprogramming.Differing from somatic cells,nucleolus undergoes distinct morphological changes during oocyte and early embryo development,while playing an indispensable role in orchestrating chromatin remodeling.1 However,the structural change and function of nucleolus in male germ cell development are still waiting for exploration.We recently reported that the role of Peter Pan Homolog(PPAN),a key rRNA processing factor,in mouse oocyte and preimplantation development.2 Here,we further constructed a male germ cell-conditional PPAN knockout(PPAN-cKO)mouse model to disturb nucleolar function in spermatogenesis.Our data showed that depletion of PPAN severely disrupts germ cell development by inducing aberrant nucleolar structure and function,resulting in an oligoasthenoteratozoospermia phenotype in mice.

关 键 词：RRNA HOMEOSTASIS IMPLANTATION 

分 类 号：Q253[生物学—细胞生物学]