四种天然抑菌物质对单增李斯特菌生物膜形成的影响  

Four Natural Antimicrobial Substances on Biofilm Formation of Listeria monocytogenes

作　　者：孙立龙 罗欣[1,2] 朱立贤 董鹏程[1] 韩广星[3] 仝林 张一敏 刘昀阁[1,2] SUN Li-long;LUO Xin;ZHU Li-xian;DONG Peng-cheng;HAN Gang-xing;TONG Lin;ZHANG Yi-min;LIU Yun-ge(Science and Engineering/Shandong Agricultural University Tai'an 271018,China;National Specialized Center of Beef Processing Technology R&D,Tai'an 271018,China;Linyi Station of National Beef Cattle and Yak Industry Technical System,Linyi 276000,China;Tongliao Station of National Beef Cattle and Yak Industry Technical System,Tongliao 028000,China)

机构地区：[1]山东农业大学食品科学与工程学院,山东泰安271018 [2]国家牛肉加工技术研发专业中心,山东泰安271018 [3]国家肉牛牦牛产业技术体系临沂站,山东临沂276000 [4]国家肉牛牦牛产业技术体系通辽站,内蒙古通辽028000

出　　处：《山东农业大学学报(自然科学版)》2025年第1期141-150,共10页Journal of Shandong Agricultural University:Natural Science Edition

基　　金：国家自然科学基金(32302181);山东省自然科学基金(ZR2022QC182);国家肉牛牦牛产业技术体系(CARS-37);山东省牛产业创新团队(SDAIT-09-09)。

摘　　要：为研究天然抑菌物质对单增李斯特菌(Listeria monocytogenes,Lm)生物膜的抑制作用,本研究首先测定了天然抑菌物质肉桂醛、丁香酚、白藜芦醇和百里醌对Lm ATCC 19115的最小抑菌浓度(minimal inhibitory concentration,MIC)和亚抑菌浓度(sub-inhibitory concentrations,SIC),并通过测定泳动能力、生物膜代谢活性(CCK-8法)和扫描电镜探究了不同浓度(MIC、1/4 MIC和1/8 MIC)下4种天然抑菌物质对该菌株的成膜能力(结晶紫染色法及活菌数法)的影响。结果表明:肉桂醛、丁香酚、白藜芦醇和百里醌对Lm ATCC 19115的MIC分别为:640、1280、400和50μg/mL,Lm ATCC 19115在4种天然抑菌物质中的SICs为1/4 MIC和1/8 MIC;上述4种天然抑菌物质均可抑制Lm ATCC 19115生物膜的形成,且在亚抑菌浓度1/4 MIC下生物膜抑制率仍可达到50%以上;4种天然抑菌物质中,肉桂醛的生物膜抑制效果最佳,其可以通过抑制Lm的泳动能力和生物膜代谢活性有效抑制Lm ATCC 19115生物膜的形成。To investigate the biofilm inhibitory effects of natural antimicrobial substances of Listeria monocytogenes(Lm),our study first determined the minimal inhibitory concentrations(MIC)and sub-inhibitory concentrations(SIC)of the natural antimicrobial substances cinnamaldehyde,eugenol,resveratrol,and thymoquinone against Lm ATCC 19115.The effects of four natural antibacterial substances on the biofilm formation ability of the strain were studied by measuring the swimming ability,biofilm metabolic activity(CCK-8 method)and scanning electron microscopy at different concentrations(MIC,1/4 MIC and 1/8 MIC).The results showed that the MICs of cinnamaldehyde,eugenol,resveratrol,and thymoquinone against Lm ATCC 19115 were 640,1280,400,and 50μg/mL,respectively.The SICs of Lm ATCC 19115 for the four natural antimicrobial substances were 1/4 MIC and 1/8 MIC.All of the above 4 natural antimicrobial substances were found to inhibit the biofilm formation of Lm ATCC 19115,with inhibition rates exceeding 50%even at the sub-inhibitory concentration of 1/4 MIC.Among the four natural antimicrobial substances,cinnamaldehyde exhibited the best biofilm inhibition effect,it effectively inhibited the biofilm formation of Lm ATCC 19115 by inhibiting its swimming motility and biofilm metabolic activity.

关 键 词：单增李斯特菌 生物膜 天然抑菌物质 亚抑菌浓度 

分 类 号：TS201.3[轻工技术与工程—食品科学]