基于代谢组学技术研究EGCG对β⁃乳球蛋白致敏性的影响  

Effect of EGCG onβ-Lactoglobulin Sensitization through Metabolomics Analysis

作　　者：余蓉[1] 李美凤[1] 王晓雅 YU Rong;LI Meifeng;WANG Xiaoya(Chengdu University of TCM,Chengdu 611137,Sichuan,China;Sichuan Institute for Drug Control,Chengdu 611731,Sichuan,China)

机构地区：[1]成都中医药大学,四川成都611137 [2]四川省药品检验研究院,四川成都611731

出　　处：《食品研究与开发》2025年第5期30-37,共8页Food Research and Development

基　　金：四川省重点研发项目(2024YFFK0155)。

摘　　要：运用代谢组学技术研究表没食子素儿茶素没食子酸酯(epigallocatechin gallate,EGCG)⁃β⁃乳球蛋白(β⁃lacto⁃globulin,βlg)复合物对βlg致过敏反应小鼠血液中代谢物的变化,筛选出过敏反应与脱敏相关的潜在生物标志物,进一步分析其代谢通路,探讨EGCG对βlg脱敏的代谢机制。采用多酚成分EGCG与βlg形成复合物进行脱敏,以βlg诱导小鼠发生过敏反应为模型组,观察空白组、βlg组、βlg⁃EGCG组小鼠过敏行为学体征,通过超高效液相色谱⁃四极杆静电场轨道阱质谱(ultra performance liquid chromatography⁃quadrupole⁃electrostatic field orbitrap mass spectrometry,UPLC⁃QE⁃MS)技术对小鼠血清进行代谢组学分析,并进一步采用主成分分析和偏最小二乘辨别分析探究各组代谢轮廓差异,筛选出差异代谢物。结果显示,βlg组和βlg⁃EGCG组的代谢谱图存在明显差异,并筛选出与过敏反应相关的40个差异代谢物,7条主要代谢通路。推测βlg致敏与EGCG脱敏作用机制可能涉及氨基酸代谢和脂类代谢等过程。A metabolomics approach was employed to investigate the changes in blood metabolites in mice withβ⁃lactoglobulin(βlg)⁃induced allergic reactions following treatment with the epigallocatechin gallate(EGCG)⁃βlg complex.Potential biomarkers related to allergic reactions and desensitization were screened,and their metabolic pathways were further analyzed to explore the metabolic mechanism of EGCG inβlg de⁃sensitization.Mice were treated with a complex of the polyphenolic components EGCG andβlg,aimed at de⁃sensitizing them toβlg⁃induced allergic reactions,and they were used as the model group.The allergic behav⁃ioral signs of the blank group,βlg group,andβlg⁃EGCG group were observed.Based on ultra performance liquid chromatography⁃quadrupole⁃electrostatic field orbitrap mass spectrometry(UPLC⁃QE⁃MS)technology,metabolomics analysis was conducted on mouse serum.Further,principal component analysis and partial least squares discriminant analysis were used to analyze the differences in metabolic profiles among groups and screen for differential metabolites.The results showed significant differences in metabolic profiles be⁃tween theβlg group and theβlg⁃EGCG group.A total of 40 differential metabolites and seven major metabolic pathways related to allergic reactions were identified.It was speculated that the mechanisms underlyingβlg sensitization and EGCG desensitization might involve processes such as amino acid metabolism and lipid me⁃tabolism.

关 键 词：食物过敏 牛奶过敏 Β-乳球蛋白 表没食子素儿茶素没食子酸酯 代谢组学 

分 类 号：TS201.2[轻工技术与工程—食品科学]