黑曲霉β-葡萄糖苷酶An3987酶法制备白藜芦醇和宝霍苷I  

Enzymatic Preparation of Resveratrol and Baohuoside I byβ-Glucosidase An3987 from Aspergillus niger

作　　者：巩宇龙 于坤朋 彭程 李利君[1,2] 倪辉 GONG Yulong;YU Kunpeng;PENG Cheng;LI Lijun;NI Hui(College of Ocean Food and Biological Engineering,Jimei University,Xiamen 361021,China;Fujian Provincial Key Laboratory of Food Microbiology and Engineering,Research Center of Food Biotechnology of Xiamen City,Xiamen 361021,China;College of Ocean Biological,Xiamen Ocean Vocational College,Xiamen 361021,China)

机构地区：[1]集美大学海洋食品与生物工程学院,福建厦门361021 [2]福建省食品微生物与酶工程重点实验室,厦门市食品生物工程技术研究中心,福建厦门361021 [3]厦门海洋职业技术学院海洋生物学院,福建厦门361021

出　　处：《现代食品科技》2025年第2期52-60,共9页Modern Food Science and Technology

基　　金：国家自然科学基金项目(31871765)。

摘　　要：β-葡萄糖苷酶在酶法制备白藜芦醇和宝霍苷I中具有重要作用。为了挖掘具有高效转化效率和对乙醇具有较好耐受性的酶资源,研究了黑曲霉CBS 513.88的β-葡萄糖苷酶An3987(XP_001394024.1)对白藜芦醇和宝霍苷I的转化率,乙醇耐受性以及pH值和温度稳定性,单因素优化其水解虎杖苷和淫羊藿苷制备白藜芦醇和宝霍苷I。研究表明β-葡萄糖苷酶An3987对虎杖苷和淫羊藿苷的酶活分别为130.02 IU/mL和70.37 IU/mL;具有较好的乙醇耐受性,10%的乙醇体积分数下对酶活力基本无影响,40%的乙醇体积分数下其仍能够保持45%的酶活力;最适温度和pH值分别为65℃和4.5。应用An3987水解虎杖苷制备白藜芦醇,65℃条件下反应时间20 min转化率为90.43%;水解淫羊藿苷制备宝霍苷I,60℃条件下反应时间40 min转化率为92.26%。该研究为黑曲霉β-葡萄糖苷酶制备白藜芦醇和宝霍苷I提供了理论基础。β-Glucosidase plays an important role in the enzymatic preparation of resveratrol and baohuoside I.In order to obtain the enzyme resources with high conversion efficiency and good ethanol tolerance,β-glucosidase An3987(XP_001394024.1)was selected from Aspergillus niger CBS 513.88 to study its conversion rate between resveratrol and baohuoside I,ethanol tolerance,pH and temperature stability,and its hydrolysis of polydatin and icariin was optimized by D-single factor experiments to prepare resveratrol and baohuoside I.The results showed that the activities ofβ-glucosidase An3987 against polydatin and icariin were 130.02 IU/mL and 70.37 IU/mL,respectively;An3987 possessed good ethanol tolerance,with 10%(V/V)ethanol having no effect on the enzyme activity of An3987,and 40%(V/V)ethanol still letting 45%enzyme activity retained;The optimum temperature and pH were 65℃and pH 4.5.Using An3987 to hydrolyze polydatin to prepare resveratrol at 65℃for 20 min,the conversion rate was 90.43%;The hydrolysis of icariin at 60℃for 40 min,the conversion rate for An3987 was 92.26%.This study provides a theoretical basis for the preparation of resveratrol and baohuoside I by theβ-glucosidase from Aspergillus niger.

关 键 词：Β-葡萄糖苷酶 乙醇耐受性 虎杖苷 白藜芦醇 淫羊藿苷 宝霍苷I 

分 类 号：TQ460.1[化学工程—制药化工]