UV-B辐射增强对苦荞叶内生微生物多样性的影响  

Effects of enhanced UV-B radiation on the diversity of endophytic microorganisms in tartary buckwheat leaves

作　　者：肖欢 杨伟亮 孔祺 龚润林 段玉莲 赵银紫 罗宗龙 冯源 leaves XIAO Huan;YANG Weiliang;KONG Qi;GONG Runlin;DUAN Yulian;ZHAO Yinzi;LUO Zonglong;FENG Yuan(College of Agriculture and Biological Sciences,Dali University,Dali 671000,China;College of Agriculture and Biological Sciences,Dali University,Key Laboratory of Agricultural Ecology of Erhai Lake Basin,Yunnan Provincial Department of Education,Dali 671000,China;College of Agriculture and Biological Sciences,Dali University,Yunnan Provincial Collaborative Innovation Center for Integrated Protection of Cangshan Mountain and Erhai Lake and Green Development of Basin,Dali 671000,China)

机构地区：[1]大理大学农学与生物科学学院,云南大理671000 [2]大理大学农学与生物科学学院,洱海流域农业生态云南省教育厅重点实验室,云南大理671000 [3]大理大学农学与生物科学学院,苍山洱海一体化保护与流域绿色发展云南省高校协同创新中心,云南大理671000

出　　处：《农业环境科学学报》2025年第2期320-329,共10页Journal of Agro-Environment Science

基　　金：云南省科技厅科技计划项目-地方高校联合专项(202001BA070001-210)。

摘　　要：利用高通量测序技术,分析UV-B(280~320nm)辐射增强对苦荞叶内生菌多样性、群落组成及功能的影响,以探讨“苦荞-内生菌”互作系统在UV-B辐射胁迫下的生态适应性,为相关研究奠定理论基础。以云荞2号为研究对象,自然光作为对照(CK),设置2个UV-B辐射强度(2.5、5.0kJ·m^(-2)),处理30d后,通过高通量测序技术分析在UV-B辐射增强条件下苦荞叶内生菌的多样性、群落组成及生物学功能变化。结果表明,在97%的序列相似性水平上,所有测序样本中苦荞叶内生真菌与内生细菌分别获得167个和425个操作分类单元(OTU)。UV-B辐射处理降低了苦荞叶内生真菌与内生细菌的总OTU数量,表现为CK>5.0kJ·m^(-2)>2.5kJ·m^(-2),但对内生菌的多样性及丰富度无显著影响(P>0.05)。3个处理组的内生真菌均以子囊菌门(Ascomycota)为优势真菌门,其相对丰度随UV-B辐射增强而升高;共有的优势细菌门为变形菌门(Proteobacteria)与厚壁菌门(Firmicutes),共有的优势细菌属为未分类产碱杆菌科(unclassified_f_Alcaligenaceae)与假单胞菌属(Pseudomonas),其相对丰度随UV-B辐射增强而显著升高。单因素相关性网络分析表明,增强UV-B辐射可促进苦荞叶内生细菌群落间的协同共生作用、削弱种间竞争,同时减弱内生真菌群落间的相互影响。对内生菌进行功能预测与分析,发现UV-B辐射增强可导致植物病原菌类功能的真菌相对丰度下降,而内生细菌功能基因中参与细胞壁/细胞膜结构的生物合成、脂质转运和代谢以及蛋白质翻译后修饰等相关基因的丰度有所上升。研究表明,UV-B辐射增强导致苦荞叶内生细菌群落组成与生物学功能发生明显改变,优势细菌群种类增加、菌群间的协同共生作用增强,这有助于提升苦荞对于UV-B辐射增强的生态适应性。This study aims to explore the mutualistic relationship between tartary buckwheat and endophytes in their ecological adaptation to UV-B radiation stress,thereby establishing a theoretical foundation for related research.Using high-throughput sequencing technology,we analyzed the effects of enhanced UV-B(280–320 nm)radiation on the diversity,community composition,and function of endophytes in tartary buckwheat leaves.The research was conducted using Yunqiao No.2 as the subject.Natural light served as the control(CK),while two UV-B radiation intensities(2.5 and 5.0 kJ·m^(-2))were applied over a period of 30 days.The diversity,community composition,and biological functions of endophytes in tartary buckwheat leaves were analyzed through high-throughput sequencing under enhanced UV-B radiation conditions.The results indicated that at a 97%sequence similarity level,167 OTUs were identified among all sequenced samples of tartary buckwheat leaf endophytes,while 425 OTUs were identified for endophytic bacteria.The UV-B radiation treatment resulted in a reduction in the total number of OTUs for both tartary buckwheat leaf endophytes and endophytic bacteria,demonstrating the following trend:CK>5.0 kJ·m^(-2)>2.5 kJ·m^(-2).However,there was no significant effect on the diversity and abundance of endophytes(P>0.05).In all three treatment groups,the endophytic fungi were predominantly Ascomycota,with their relative abundance increasing alongside enhanced UV-B radiation.The dominant bacterial phyla included Proteobacteria and Fusobacteria,with Proteobacteria and Firmicutes being the common dominant phyla.The prevalent bacterial genera were unclassified_f_Alcaligenaceae and Pseudomonas,both of which showed a significant increase in relative abundance with enhanced UV-B radiation.One-way correlation network analysis revealed that increased UV-B radiation could promote synergistic symbiosis among endophytic bacterial communities in tartary buckwheat leaves,while weakening interspecific competition and interactions among endoph

关 键 词：UV-B辐射 苦荞 高通量测序 内生菌 微生物多样性 

分 类 号：S517[农业科学—作物学]