载槲皮素复合纳米颗粒制备及稳定性和缓释性能  

Preparation,stability and slow release performance of quercetin loaded composite nanoparticles

作　　者：刘志军[1] 秦薪博 李志义[1] 刘凤霞[1] 许晓飞[1] 魏炜[1] LIU Zhijun;QIN Xinbo;LI Zhiyi;LIU Fengxia;XU Xiaofei;WEI Wei(R&D Institute of Fluid and Powder Engineering,Dalian University of Technology,Dalian 116024,Liaoning,China)

机构地区：[1]大连理工大学流体与粉体工程研究设计所,辽宁大连116024

出　　处：《精细化工》2025年第3期611-619,629,共10页Fine Chemicals

摘　　要：以槲皮素(Que)和玉米醇溶蛋白(Zein)为核、酪蛋白酸钠(SC)或SC-海藻酸钠(SA)为壳,采用抗溶剂沉淀法和静电吸附法制备了SC单壳层载槲皮素复合纳米颗粒(Q-Z-SC)和SC-SA双壳层载槲皮素复合纳米颗粒(Q-Z-SC-SA)。在m(Zein)∶m(SC)=1∶1条件下,分别考察了m(Que)∶m(Zein)、m(SC)∶m(SA)对Q-Z-SC、Q-Z-SC-SA的粒径分布、Zeta电位、Que包埋率的影响。采用FTIR探究了Q-Z-SC和Q-Z-SC-SA的形成机制。通过稳定性和体外模拟释放实验,分析了pH和离子强度(NaCl溶液浓度)对Q-Z-SC、Q-Z-SC-SA稳定性的影响和模拟胃肠条件的Que释放情况。结果表明,由m(Que)∶m(Zein)∶m(SC)=1∶25∶25制备的Q-Z-SC(Q_(1)-Z_(25)-SC_(25))的平均粒径为158.2 nm,Que包埋率为79.53%;由m(Que)∶m(Zein)∶m(SC)=1∶25∶25、m(SC)∶m(SA)=25∶7.50制备的Q-Z-SC-SA(Q_(1)-Z_(25)-SC_(25)-SA_(7.5))的平均粒径为251.6 nm,Que包埋率高达90.71%。静电、氢键和疏水相互作用是复合纳米颗粒形成的主要作用力。Q_(1)-Z_(25)-SC_(25)和Q_(1)-Z_(25)-SC_(25)-SA_(7.5)均具有优异的pH和离子强度稳定性。Q_(1)-Z_(25)-SC_(25)和Q_(1)-Z_(25)-SC_(25)-SA_(7.5)在模拟胃消化阶段的Que释放率分别为87.23%和69.40%,在模拟肠消化阶段的Que释放率分别为11.04%和22.64%。Sodium caseinate(SC)single shell quercetin nanoparticles(Q-Z-SC)and SC-sodium alginate(SA)double shell quercetin nanoparticles(Q-Z-SC-SA)were prepared by antisolvent precipitation and electrostatic adsorption method using quercetin(Que)and zein(Zein)as core,and SC or SC-SA as shell.The effects of m(Que)∶m(Zein)and m(SC)∶m(SA)on particle size distribution,Zeta potential and Que embedding rate of Q-Z-SC and Q-Z-SC-SA with m(Zein)∶m(SC)=1∶1 were analyzed,with the formation mechanism explored by FTIR.And the influence of pH and ionic strength(NaCl solution concentration)on the stability of Q-Z-SC,Q-Z-SC-SA and Que release under simulated gastrointestinal conditions were evaluated by stability and in vitro simulated release experiments.The results demonstrated that the mean particle size and Que embedding rate of Q-Z-SC(Q_(1)-Z_(25)-SC_(25))prepared from m(Que)∶m(Zein)∶m(SC)=1∶25∶25 was 158.2 nm and 79.53%,respectively,while those of Q-Z-SC-SA(Q_(1)-Z_(25)-SC_(25)-SA_(7.5))prepared from m(Que)∶m(Zein)∶m(SC)=1∶25∶25 and m(SC)∶m(SA)=25∶7.50 were 251.6 nm and 90.71%.The formation of composite nanoparticles was driven by electrostatic,hydrogen bonding and hydrophobic interactions.Both Q_(1)-Z_(25)-SC_(25) and Q_(1)-Z_(25)-SC_(25)-SA_(7.5) exhibited excellent pH and ionic strength stabilities,as well as the capacity to regulate the release of Que under simulated gastrointestinal conditions.Que release rates of Q_(1)-Z_(25)-SC_(25) and Q_(1)-Z_(25)-SC_(25)-SA_(7.5) in simulated gastric digestion stage were 87.23%and 69.40%,respectively,while 11.04%and 22.64%,respectively,in simulated intestinal digestion stage.

关 键 词：槲皮素 玉米醇溶蛋白 酪蛋白酸钠 海藻酸钠 纳米颗粒 食品化学品 

分 类 号：TQ469[化学工程—制药化工]