母婴来源植物乳杆菌的体外益生特性及安全性评价  

Evaluation of in vitro probiotic characteristics and safety of Lactobacillus plantarum isolated from breast milk and infant feces

作　　者：徐晶晶 于俊娟 严浩东 张臣臣 康文丽 潘丽娜 汪家琦 李威 顾瑞霞[1,2] XU Jingjing;YU Junjuan;YAN Haodong;ZHANG Chenchen;KANG Wenli;PAN Lina;WANG Jiaqi;LI Wei;GU Ruixia(College of Food Science and Engineering,Yangzhou University,Yangzhou 225000,China;Jiangsu Key Laboratory of Dairy Biotechnology and Safety Control,Yangzhou 225000,China;Ausnutria Dairy Corporation Ltd.,Changsha 410127,China;Hunan Engineering Research Center of Human Microecological Products,Changsha 410127,China)

机构地区：[1]扬州大学食品科学与工程学院,江苏扬州225000 [2]扬州大学,江苏省乳品生物技术与安全控制重点实验室,江苏扬州225000 [3]澳优乳业(中国)有限公司,湖南长沙410127 [4]人体微生态制品湖南省工程研究中心,湖南长沙410127

出　　处：《食品与发酵工业》2025年第6期144-151,共8页Food and Fermentation Industries

基　　金：湖南省科技创新计划资助项目(2022RC1063);扬州大学“青蓝工程”;扬州市-扬州大学市校合作共建科技创新平台项目(YZ2020265);湖南省长沙市望城区科技计划重点研发计划项目(K221012);扬州大学“高端人才支持计划”项目。

摘　　要：为筛选适用于婴幼儿的候补益生菌,该研究从母乳和婴儿粪便中分离乳酸菌。利用耐胆盐能力对其进行初筛,并针对耐酸能力、模拟胃肠液耐受能力、Caco-2肠上皮细胞黏附能力、抑菌能力、溶血能力、产生物胺能力和耐药性对其体外益生特性及安全性进行评价。研究共分离到131株母婴源乳酸菌,其中4株菌的耐胆盐能力较强,在1 g/L胆盐中的存活率均大于45%,经鉴定均为植物乳杆菌。4株菌在pH值2.5模拟胃液中的存活率均大于50%。其中,M664在模拟胃肠液中的存活率达到49.32%。应激消化后,M678对Caco-2细胞的黏附率达到10.98%。4株菌对6种腐败菌和致病菌的抑菌圈直径均大于16 mm,不溶血,不产生物胺,对大多数抗生素表现敏感。总体而言,该研究得到的植物乳杆菌有别于西方膳食模式国家的母婴来源乳杆菌,植物乳杆菌M664有较强的胃肠道环境耐受能力,植物乳杆菌M678有更好的抑菌能力和细胞黏附能力,2株菌表现出更为优良的益生潜力和安全性,是国人婴幼儿适用的优良益生菌候补菌株。To screen candidate strains of probiotics for infants,lactic acid bacteria(LAB)strains were isolated from breast milk and infant feces and screened according to bile salt tolerance.Then the strains were explored by investigating acid tolerance,simulated gastrointestinal fluid tolerance,adhesion ability,bacteriostatic ability,hemolytic ability,amine production ability,and antibiotic resistance.A total of 131 strains of LAB were isolated,among which four strains showed better bile salt tolerance.The survival rates of all these four strains were higher than 45%under 0.1%bile salt,and all these strains belonged to Lactobacillus plantarum.The survival rates of these strains were higher than 50%under pH_(2).5 simulated gastric juice.The survival rate of M664 under simulated gastrointestinal fluid was 49.32%.The adhesion rate of digested M678 cells to Caco-2 cells was 10.98%.The diameters of the bacteriostatic circle of the four strains to the six spoilage bacteria and pathogens were all larger than 16 mm.In addition,these strains did not produce biological amines and were sensitive to most antibiotics.Overall,L.plantarum strains obtained in this study were different from Lactobacillus isolated from breast milk and infant feces from Western dietary pattern countries.L.plantarum M664 had better gastrointestinal environmental tolerance,and L.plantarum M678 had better antibacterial ability and cell adhesion ability.These two strains showed better probiotic potential and safety and were excellent candidate strains of probiotics for Chinese infants.

关 键 词：母乳 婴儿粪便 植物乳杆菌 安全性评价 

分 类 号：TS201.3[轻工技术与工程—食品科学]