发酵豆渣上清液对高脂HepG2细胞糖脂代谢的影响  

Effects of Fermented Okara Supernatant on Glycolipid Metabolism in High-fat HepG2 Cells

作　　者：肖宇仪 李鹏程[1] 刘子琦 王钰博 王秀娟[1] 朴春红 XIAO Yuyi;LI Pengcheng;LIU Ziqi;WANG Yubo;WANG Xiujuan;PIAO Chunhong(College of Food Science and Engineering,Jilin Agricultural University,Changchun 130118;College of Food and Pharmaceutical Engineering,Wuzhou University,Wuzhou 543002,Guangxi)

机构地区：[1]吉林农业大学食品科学与工程学院,长春130118 [2]梧州学院食品与制药工程学院,广西梧州543002

出　　处：《中国食品学报》2025年第2期79-91,共13页Journal of Chinese Institute Of Food Science and Technology

基　　金：吉林省科技发展计划项目(20210202107NC);财政部和农业农村部:国家现代农业产业技术体系项目(CARS-04)。

摘　　要：为减少豆渣资源的浪费,研究发酵豆渣上清液(FOS)的功能活性。以马克斯克鲁维酵母C21为发酵菌株,发酵豆渣制备FOS。在分析FOS活性成分的基础上,评价其抗氧化能力和体外改善糖脂代谢紊乱的效果及机制。结果表明,FOS中可溶性总糖、游离氨基酸和总酚的含量显著升高,相较于未发酵豆渣上清液(NFOS)分别提高了13.52%,507.95%和16.67%,且总抗氧化能力为8.57μmol Fe^(2+)/mL,比发酵前提升了39.15%。油酸诱导的高脂HepG2细胞试验结果表明,与NFOS相比,FOS的干预显著降低了细胞中的脂滴数量以及甘油三酯(25.39%)和总胆固醇(26.90%)含量,提高了细胞的葡萄糖消耗量(46.77%)和胞内糖原(51.29%)含量。免疫印迹结果显示,400μg/mL FOS能够显著上调糖脂代谢相关蛋白的表达,PPARα、IRS1和GLUT4与NFOS相比分别上调了30.89%,127.16%和22.84%。结论:FOS能够显著改善油酸诱导的HepG2细胞糖脂代谢紊乱,通过激活PPARα、IRS1和GLUT4蛋白的表达,减少细胞内的脂质蓄积并增强细胞的葡萄糖摄取能力,说明FOS在预防和改善肥胖引起的高脂血症和2型糖尿病方面具有潜在作用。To reduce the waste of okara resources and to investigate the functional activities of fermented okara supernatant(FOS).FOS was prepared from fermented okara with Kluyveromyces marxianus C21 as the fermentation strain.Based on the analysis of the active components of FOS,the antioxidant capacity and in vitro improvement of glycolipid metabolism disorders were evaluated,and the mechanism of FOS was also evaluated.The results showed that the contents of total soluble sugar,free amino acid,and total phenol in FOS were significantly increased compared with those of NFOS(non-fermented okara supernatant)by 13.52%,507.95%,and 16.67%,respectively,and the total antioxidant capacity was 8.57μmol Fe^(2+)/mL,which was enhanced by 39.15%compared with that before the fermentation.The experimental results of oleic acid-induced high-fat HepG2 cells showed that the intervention of FOS significantly reduced the lipid droplet number as well as the content of triglyceride(25.39%)and total cholesterol(26.90%),and increased the glucose consumption(46.77%)and intracellular glycogen(51.29%)content of the cells,in comparison with that of NFOS.Western blot results showed that 400μg/mL FOS significantly up-regulated the expression of glycolipid metabolism-related proteins,and PPARα,IRS1,and GLUT4 were up-regulated by 30.89%,127.16%,and 22.84%,respectively,compared with NFOS.Overall,FOS significantly ameliorated oleic acid-induced glycolipid metabolism disorders in HepG2 cells,reducing intracellular lipid accumulation and enhancing cellular glucose uptake by activating the expression of PPARα,IRS1,and GLUT4 proteins.The results showed that FOS has a potential role in preventing and ameliorating obesity-induced hyperlipidemia and type 2 diabetes.

关 键 词：发酵豆渣上清液 马克斯克鲁维酵母 抗氧化 HEPG2细胞 糖脂代谢 

分 类 号：TS210.9[轻工技术与工程—粮食、油脂及植物蛋白工程] TS201.4[轻工技术与工程—食品科学与工程]