草原羊脑磷脂型DHA对小鼠生理机能的影响  

Effect of phospholipid-type DHA from grassland sheep brain on physiological functions of mice

作　　者：包音都古荣·金花[1,2,3] 宝鲁日 和硕特麦丽斯 侯荣伦 呼格吉勒图 敖日格勒 岩松 敖日格乐 周欢敏 BAOYINDUGURONG·Jinhua;BAOLURI;HESHUOTE Mailisi;HOU Ronglun;HUGEJILETU;AORIGELE;YAN Song;AORIGELE;ZHOU Huanmin(College of Food Science and Engineering,Inner Mongolia Agricultural University,Hohhot 010018,China;Inner Mongolia Key Laboratory of Biofabrication,Hohhot 010010,China;Inner Mongolia Hesige Agriculture and Animal Husbandry Technology Co.,Ltd.,Xilingol League 026321,Inner Mongolia,China;Department of Pathology,School of Basic Medical Sciences,Inner Mongolia Medical University,Hohhot 010110,China;Crystal Pharmatech,Inc.,New Jersey 08512,USA)

机构地区：[1]内蒙古农业大学食品科学与工程学院,呼和浩特010018 [2]内蒙古生物制造重点实验室,呼和浩特010010 [3]内蒙古贺斯格农牧业科技有限公司,内蒙古锡林郭勒盟026321 [4]内蒙古医科大学基础医学院病理教研室,呼和浩特010110 [5]晶云药物科技股份有限公司,美国新泽西08512

出　　处：《中国油脂》2025年第3期60-67,共8页China Oils and Fats

基　　金：内蒙古自治区自然科学基金项目(2023MS03034);内蒙古自治区科技计划项目(2023YFDZ0050);内蒙古自治区科技计划项目(2021GG0348);草原英才项目(内组通字[2021]333号,内人才发[2023]3号)。

摘　　要：旨在为草原羊脑磷脂型DHA(DHA-PLs)的开发利用提供科学依据,从苏尼特羊脑中萃取DHA-PLs,在确定其不携带朊病毒蛋白的基础上,选择7周龄、平均体质量为27 g的SPF级昆明雌性小白鼠30只,分为空白组(基础饲料)、鱼油组(基础饲料+鱼油)和羊脑DHA-PLs组(基础饲料+羊脑DHA-PLs),考察饲喂3个月后各组小鼠体征指标、周能量排出量、粪便中粗脂肪含量和脏器指数的变化,测定其血清生化和肝功能指标,观察肠道和脏器组织形态,以揭示羊脑DHA-PLs对小鼠营养物质吸收、脏器、血脂和肝功能的影响程度。结果表明:羊脑中无朊病毒蛋白,正常朊蛋白含量最高达200 mg/g;3个月饲喂试验结束后,各组小鼠健康状况良好,平均日采食量无差异,终体质量均显著增大(p<0.05);与空白组比较,羊脑DHA-PLs组小鼠周能量排出量,粪便中粗脂肪含量,血清中甘油三酯和总胆固醇含量,以及谷草转氨酶、谷丙转氨酶水平显著降低(p<0.05),但与鱼油组无显著差异(p>0.05);各组小鼠脏器指数之间无显著差异(p>0.05),心脏、肝脏、肾脏、脾脏、胃和脑等组织均未出现病变,肠道均无损伤,空肠绒毛高度与隐窝深度比值组间无差异(p>0.05),但羊脑DHA-PLs组和鱼油组小鼠肠道分别长出14个和13个潘氏斑,而空白组未出现。综上,草原羊脑DHA-PLs能促进小鼠营养物质吸收,显著降低血脂,提高肝脏机能和肠道免疫机能,对小鼠脏器无不良影响,可以应用于食品和保健品生产中。Aiming to provide scientific basis for the development and utilization of grassland sheep brain phospholipid-type DHA(DHA-PLs),the DHA-PLs was extracted from Sunite sheep brains.Based on the determination that the DHA-PLs did not carry prion protein,a total of 30 seven-week-old SPF Kunming female mice an average body mass of 27 g were selected and divided into blank group(basal feed),fish oil group(basal feed added with fish oil),and sheep brain DHA-PLs group(basal feed added with sheep brain DHA-PLs).The changes in physical signs,weekly energy discharge,crude fat content in feces,and organ indexes of the mice fed for 3 months were studied,and their serum biochemistry and liver function indexes were determined,as well as intestinal and organ tissue morphology were observed to reveal the degree of the effects of sheep brain DHA-PLs on nutrient absorption,organs,blood lipids and liver function in mice.The results showed that no prion protein in the sheep brain,and the highest normal prion protein level reached up to 200 mg/g.At the end of the 3-month feeding test,the three groups of mice were in good health,with no difference in average daily feed intake,and all of them had significantly larger final body mass(p<0.05).Compared with the blank group,the weekly energy discharge,crude fat content in feces,serum triglyceride and cholesterol contents,and the levels of aspartate aminotransferase and alanine of mice in the sheep brain DHA-PLs group were significantly reduced(p<0.05),but there was no significant difference with the fish oil group(p>0.05).There were no significant differences in organ indexes among the mice in the three groups(p>0.05),and the tissues of the heart,liver,kidneys,spleen,stomach,and brain did not show any lesions.There was no injury to the intestines of the mice in the three groups,and the ratio of villus height to crypt depth in the jejunum showed no difference between the three groups(p>0.05),but in the sheep brain DHA-PLs group and the fish oil group,the intestines of mice grew 14 and 13 Peye

关 键 词：草原羊脑磷脂型DHA 脏器指数 血脂 肝功能 组织学分析 潘氏斑 

分 类 号：TS201.4[轻工技术与工程—食品科学] TS225.2[轻工技术与工程—食品科学与工程]