海洋胶红酵母H118发酵生产类胡萝卜素研究  

Fermentation production of carotenoids with the marine Rhodotorula mucilaginosa H118

作　　者：程仕伟[1] 仉倩 王玉芳 张佳雯 孙嘉蔚 陈卫卫 鲁仪增 CHENG Shiwei;ZHANG Qian;WANG Yufang;ZHANG Jiawen;SUN Jiawei;CHEN Weiwei;LU Yizeng(School of Life Sciences,Ludong University,Yantai 264025,China;Department of Information Engineering,Yantai Vacational College,Yantai 264670,China;Shandong Forest and Grass Germplasm Resources Center,Jinan 250102,China)

机构地区：[1]鲁东大学生命科学学院,山东烟台264025 [2]烟台职业学院信息工程系,山东烟台264670 [3]山东省林草种质资源中心,山东济南250102

出　　处：《发酵科技通讯》2025年第1期14-17,51,共5页Bulletin of Fermentation Science and Technology

基　　金：烟台市科技计划发展项目(2022XDRH029);山东省重点研发计划(重大科技创新工程)项目(2021LZGC02304)。

摘　　要：类胡萝卜素作为一类天然色素具有广泛的应用,而红酵母发酵生产类胡萝卜素具有独特的优势。以海洋源胶红酵母H118为研究对象,采用Plackett-Burman实验设计对影响类胡萝卜素产生的9个因素进行统计分析,影响极显著的因素为培养温度,对其进行梯度优化后确定为25℃。在此基础上,采用优化的工艺条件经5 L发酵罐批培养,发酵至36 h和48 h的类胡萝卜素质量浓度分别达1591.11,1627.92μg/L,该结果可为后续类胡萝卜素产业化生产及其应用提供技术支撑。Carotenoids are widely used as natural pigments,and Rhodotorula has unique advantages for producing carotenoids through fermentation.The marine Rhodotorula mucilaginosa H118 was used as the research subject,and the nine factors affecting the production of carotenoids were statistically analyzed with the Plackett-Burman experimental design,and the culture temperature was identified as the most significant factor and it was optimized to 25℃through gradient optimization.Based on this,the optimized process conditions of Rhodotorula mucilaginosa H118 were adopted for batch culture in a 5 L fermenter,and the carotenoid content reached 1591.11μg/L and1627.92μg/L with fermentation for 36 h and 48 h,respectively,providing technical support for the subsequent large-scale production and application of carotenoids.

关 键 词：胶红酵母 类胡萝卜素 发酵 生物量 

分 类 号：Q939.97[生物学—微生物学]