60份广西茶树种质资源的染色体倍性鉴定  

Chromosome Ploidy Identification of 60 Germplasm Resources of Tea Plants in Guangxi

作　　者：黄寿辉 彭靖茹[1] 温立香[1] 张芬[1] 陈家献 袁冬寅 欧淑琼 张栩浩 赵媛[1] HUANG Shou-hui;PENG Jing-ru;WEN Li-xiang(Gungaxi Subtropical Crops Research Institute,Nanning,Guangxi 530001)

机构地区：[1]广西壮族自治区亚热带作物研究所,广西南宁530001

出　　处：《安徽农业科学》2025年第5期10-13,17,共5页Journal of Anhui Agricultural Sciences

基　　金：广西重点研发计划项目(桂科AD18281068);广西重点研发计划项目(桂科AB19245006);国家现代农业产业技术体系茶叶南宁综合试验站(nycytxgxcxtd-18-06);广西农科院2021—2025年稳定资助科研团队项目(桂科农2021YT145);科技先锋队“强农富民”“六个一”专项行动项目(桂农科盟202206-2)。

摘　　要：基于流式细胞仪对植物染色体倍性鉴定的高效性和准确性,利用流式细胞仪分2批次对60份广西茶树种质资源的染色体倍性进行鉴定分析,以期得到种质资源准确的染色体倍性信息,为茶树育种提供科学依据。共鉴定出茶树二倍体53份,三倍体6份,单倍体1份,未检测到四倍体。而检测到的单倍体茶树180(龙脊古树2号)在后续的茶树染色体加倍获得多倍体育种提供原始材料,为茶树的多倍体育种和运用研究提供参考。Given the efficiency of flow cytometry in determining plant ploidy,in this study,flow cytometry was employed to identify the ploidy of 60 tea tree germplasm resources in Guangxi,aiming to obtain accurate ploidy data to support tea plant breeding.The result showed that a to-tal of 53 diploids,6 triploids,and 1 haploid were identified,but no tetraploid was detected.The identified haploid tea tree 180(Longji Ancient Tea Tree No.2)will serve as the original material for subsequent tea plant chromosome doubling to produce polyploid breeding materials,thus providing a reference for the breeding and utilization of polyploid tea trees.

关 键 词：茶树 种质资源 染色体倍性 流式细胞术 鉴定 

分 类 号：S571.1[农业科学—茶叶生产加工]