基于非靶向代谢组学揭示酸茶水提物对广式香肠抗氧化活性的影响  

Revealing Effect of Sour Tea Water Extract on Antioxidant Activity of Cantonese Sausages Based on Non-Targeted Metabolomics

作　　者：李国友 肖蓉[1] 黄元相 杨卫星[1] 朱绍先 侯艳[3] LI Guo-you;XIAO Rong;HUANG Yuan-xiang;YANG Wei-xing;ZHU Shao-xian;HOU Yan(College of Food Science and Technology,Yunnan Agricultural University,Kunming 650201,China;Kunming Gaoshanggao Food Co.,Ltd.,Kunming 650201,China;College of Tea,Yunnan Agricultural University,Kunming 650201,China)

机构地区：[1]云南农业大学食品科学技术学院,昆明650201 [2]昆明高上高食品有限公司,昆明650201 [3]云南农业大学茶学院,昆明650201

出　　处：《中国调味品》2025年第3期23-31,共9页China Condiment

基　　金：云南省科技重大专项(202002AA100005);云南德凤茶业有限公司合作项目(KX141721)。

摘　　要：利用植物中所含的天然抗氧化剂代替合成抗氧剂延迟肉制品氧化已成为当前的研究热点。课题组前期研究发现德昂族酸茶中含有大量的抗氧化活性物质,但对广式香肠的抗氧化活性是否存在影响鲜见报道。该研究在广式香肠制作过程中添加0.5%酸茶水提物,参照相关国家标准对广式香肠储藏过程中的酸价、过氧化值(POV)、硫代巴比妥酸反应物(TBARS)值进行动态监测,同时利用非靶向代谢组学技术检测其储藏过程中的差异代谢物,并分析其涉及的代谢通路。结果表明,添加酸茶水提物的广式香肠储藏过程中第0,30,60,90天的酸价、POV、TBARS值均显著低于对照组(P<0.05)。UPLC-MS/MS共筛选出187种差异代谢物,主要涉及32条代谢通路,其中D-谷氨酰胺-D-谷氨酸代谢(D-glutamine and D-glutamate metabolism)和甘油磷脂代谢(glycerophospholipid metabolism)最显著。D-谷氨酰胺和D-谷氨酸代谢通路涉及两种差异代谢物,分别为D-谷氨酰胺(D-glutamine)和L-谷氨酰胺(L-glutamine),两种物质在第90天显著下降(P<0.05);甘油磷脂代谢通路涉及4种差异代谢物,分别为二羟丙酮磷酸(dihydroxyacetone phosphate)、乙醇胺磷酸酯(O-phosphoethanolamine)、2-胺乙基-2,3-二羟丙基-羟基磷酸酯(glycerylphosphory lethanolamine)和甘油磷酰胆碱(glycerophosphocholine)。其中,2-胺乙基-2,3-二羟丙基-羟基磷酸酯和甘油磷酰胆碱显著上升(P<0.05),二羟丙酮磷酸和乙醇胺磷酸酯显著下降(P<0.05),表明酸茶可能通过调节香肠中的脂类代谢、氨基酸代谢发挥抗氧化作用。The use of natural antioxidants in plants instead of synthetic antioxidants to delay the oxidation of meat products has become a current research hotspot.Through the preliminary research of the research group,it is found that De'ang sour tea contains a large amount of antioxidant active substances,but there are few reports on whether they have an effect on the antioxidant activity of Cantonese sausages.In this study,during the production of Cantonese sausages,0.5%sour tea water extract is added,and the acid value,peroxide value(POV)and thiobarbituric acid reactive substance(TBARS)value during storage are dynamically monitored according to relevant national standards.At the same time,non-targeted metabolomics is used to detect differential metabolites during storage and analyze the metabolic pathways involved.The results show that the acid value,POV and TBARS value of Cantonese sausages added with sour tea water extract stored at the 0th,30th,60th,90th days are significantly lower than those of the control group(P<0.05).A total of 187 differential metabolites are screened by UPLC-MS/MS,mainly involving 32 metabolic pathways,among which,D-glutamine and D-glutamate metabolism and glycerophospholipid metabolism are the most significant.The two metabolites involved in the D-glutamine and D-glutamine metabolic pathways are D-glutamine and L-glutamine,which are significantly decreased on the 90th day(P<0.05).The glycerophospholipid metabolic pathway involves four differential metabolites,which are dihydroxyacetone phosphate,O-phosphoethanolamine,glycerylphosphory lethanolamine and glycerophosphocholine respectively.Among them,glycerylphosphory lethanolamine and glycerophosphocholine are significantly increased(P<0.05),while dihydroxyacetone phosphate and O-phosphoethanolamine are significantly decreased(P<0.05),indicating that sour tea may exert antioxidant effects by regulating lipid metabolism and amino acid metabolism in sausages.

关 键 词：酸茶 广式香肠 抗氧化性 代谢组学 

分 类 号：TS251.65[轻工技术与工程—农产品加工及贮藏工程]