Methionine supplementation regulates eggshell quality and uterine transcriptome in late-stage broiler breeders  

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作  者:Mingkun Gao Youying Chen Xiaomin Li Dongli Li Aiqiao Liu Lu Gong Zhonghua Ning Wei Nie Yuming Guo Zengpeng Lv 

机构地区:[1]State Key Laboratory of Animal Nutrition and Feeding,College of Animal Science and Technology,China Agricultural University,Beijing 100193,China [2]Beijing Huadu Yukou Poultry Industry Co.,Ltd.,Beijing 101206,China

出  处:《Animal Nutrition》2024年第4期56-69,共14页动物营养(英文版)

基  金:funded by the National Key R&D Program of China(2021YFD1300404);the China Agriculture Research System program(CARS-40 and CARS-41);the Beijing Natural Science Foundation(NO.6222036);the Meat Chicken Science and Technology Backyard Program in Dongchengfang Town,Zhuozhou,Hebei Province,China(CAUZ2024013).

摘  要:This study aimed to compare the effects of dietary methionine(Met)and 2-hydroxy-4-(methylthio)-butanoate(HMTBA)on the eggshell quality of broiler breeder hens and elucidate the mechanism of Met in improving eggshell quality from the perspectives of eggshell microstructure and shell gland physiological function.A total of 720 WOD188 broiler breeder hens at 40 weeks old were assigned to 3 groups,with 8 replicates per group and 30 birds per replicate.Over 7 weeks,birds were fed a basal diet or the same diet supplemented with 0.15%Met or 0.17%HMTBA.Our findings revealed significant improvements in the Met group for egg shape index,shell thickness,breaking strength,and fracture toughness(P<0.05),whereas the HMTBA group showed no significant improvements(P>0.05).Met supplementation increased calcium and phosphorus levels in both serum and shell gland tissue(P<0.05),and enhanced Ca^(2+) ATPase activity in shell gland tissue(P<0.05).Histomorphological changes cluded enhanced mucosal fold dimensions and increased epithelial height in the shell gland(P<0.05).Met also improved eggshell ultrastructure,resulting in a thicker effective layer and broader mammillae with fewer type B structures(P<0.05).The mRNA levels for genes regulating eggshell ultrastructure,such as ovocleidin-116(OC-116),calbindin 1(CALB1),and integral membrane protein 2C(ITM2C),were significantly upregulated in the Met group(P<0.05).Transcriptome analysis identified 248 differentially upregulated genes in the Met group,primarily linked to the non-canonical Wnt/Ca^(2+) signaling pathway,crucial for calcium ion transport and cellular proliferation.This research highlights that Met supplementation improves eggshell quality by enhancing calcium transport and cellular proliferation in uterine function,particularly through the modulation of Wnt family member 11(WNT11)and CALB1,influencing calcium deposition and ultrastructural development.

关 键 词:Eggshell quality Eggshell ultrastructure METHIONINE Broiler breeder TRANSCRIPTOME 

分 类 号:R73[医药卫生—肿瘤]

 

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