Saccharomyces cerevisiae and Kluyveromyces marxianus yeast co-cultures modulate the ruminal microbiome and metabolite availability to enhance rumen barrier function and growth performance in weaned lambs  

作　　者：Zixuan Xu Lan Yang Hui Chen Shixiong Liu Xueqiang Li Songjian Li Chun Ying Xiao Li Rui Du Dacheng Liu 

机构地区：[1]College of Veterinary Medicine,Inner Mongolia Agricultural University,Hohhot 010018,China

出　　处：《Animal Nutrition》2024年第4期139-152,共14页动物营养(英文版)

基　　金：supported by the National Key Research and Development Program of China(2023YFE0100400);National Natural Science Foundation of China(32060774);Science and Technology Project of Inner Mongolia Autonomous Region(2020GG0036);Basic Scientific Research Business Project of Universities directly under the Inner Mongolia Autonomous Region(BR22-11-17).

摘　　要：In lambs,weaning imposes stress that can contribute to impaired rumen epithelial barrier functionality and immunological dysregulation.In this study,the effects of a yeast co-culture consisting of Saccharomyces cerevisiae and Kluyveromyces marxianus(NM)on rumen health in lambs was evaluated,with a focus on parameters including growth performance,ruminal fermentation,and epithelial barrier integrity,ruminal metabolic function,and the composition of the ruminal bacteria.In total,24 lambs were grouped into four groups of six lambs including a control(C)group fed a basal diet,and N,M,and NM groups in which lambs were fed the basal diet respectively supplemented with S.cerevisiae yeast cultures(30 g/d per head),K.marxianus yeast cultures(30 g/d per head),and co-cultures of both yeasts(30 g/d per head),the experiment lasted for 42 d.Subsequent analyses revealed that relative to the C group,the average daily gain(ADG)of lambs in the NM group was significantly greater and exhibited significant increases in a range of mRNA relative expression including monocarboxylate transporter 1(MCT1),(Na^(+))/hydrogen(H^(+))exchanger 1(NHE1),(Na^(+))/hydrogen(H^(+))exchanger 3(NHE3),protoncoupled amino acid transporter 1(PAT1),vacuolar H^(+)-ATPase(vH+ ATPase),claudin-1,occludin in the rumen epithelium(P<0.05).Compared with the C group,the pH of the rumen contents in the NM group was significantly decreased,and the concentrations of acetate,propionate,and butyrate were significantly increased(P<0.05).Analysis of the rumen bacteria showed that the NM group exhibited increases in the relative abundance of Prevotella,Treponema,Moryella,Fibrobacter,CF231 and Ruminococcus(P<0.05).Metabolomics analyses revealed an increase in the relative content of phthalic acid and cinnamaldehyde in the NM group as compared to the C group(P<0.05),together with the greater relative content of L-tyrosine,L-dopa,rosmarinic acid,and tyrosol generated by the tyrosine metabolic pathway(P<0.05).Spearman's correlation analyses revealed relative abundance levels o

关 键 词：Weaned lamb Rumen bacteria CO-CULTURE Metabolic profile Rumen function 

分 类 号：R73[医药卫生—肿瘤]