Rauvolfa vomitoria phenol extract relieves pentylenetetrazol-induced seizures in Swiss mice and protects some temporal lobe structures  

作　　者：Moses B.Ekong Okokon O.Bassey Deborah I.Ebeh Godslove D.Usukuma Darlington C.Samuel Rosemary B.Bassey Aniekan I.Peter Christopher C.Mbadugha Jude E.Okokon Monday I.Akpanabiatu 

机构地区：[1]Department of Anatomy,Faculty of Basic Medical Sciences,University of Uyo,PMB 1017,Uyo,Nigeria [2]Department of Science Education,Donald and Barbara Zucker School of Medicine at Hofstra/Northwell,Hempstead,NY 11549,USA [3]Department of Pharmacology and Toxicology,Faculty of Pharmacy,University of Uyo,PMB 1017,Uyo,Nigeria [4]Department of Biochemistry,Faculty of Sciences,University of Uyo,PMB 1017,Uyo,Nigeria

出　　处：《Acta Epileptologica》2024年第4期303-317,共15页癫痫学报(英文)

基　　金：supported in part by the Tertiary Education Trust Fund(TETFund).The International Brain Research Organization(IBRO)Early Career Grant and the International Society for Neurochemistry(ISN)Committee for Aid and Education in Neurochemistry(CAEN)provided the funding for small equipment.

摘　　要：Background Rauvolfa vomitoria(R.vomitoria)is a plant of economic importance due to its diverse ethnomedicinal properties,including the anticonvulsant efect.In this study,we studied the antiseizure and neuroprotective potentials of R.vomitoria extracts against pentylenetetrazol(PTZ)-induced kindling.Methods Twenty-fve adult Swiss mice(25-30 g)were assigned to fve groups(n=5):control group,PTZ treatment group,and PTZ treatment after receiving oral R.vomitoria crude extract(100 mg/kg),R.vomitoria phenol extract(50 mg/kg)or sodium valproate(15 mg/kg)every 48 h for 28 days.Seizure scores,cognitive behavioral tests including novel object test,Y-maze test,and the elevated plus maze test,as well as brain neurochemicals and histomorphology studies,were performed.Results Compared with the control group,the PTZ group showed comparable body weight and durations in closed and open arms(P>0.05),but preference for familiar objects,signifcant(P<0.05)spontaneous alternation,increased monoamine oxidase activity and nitric oxide level,and Nissl chromatolysis in the temporal lobe structures including the cortex,hippocampus,and amygdala.R.vomitoria phenol extract pretreatment signifcantly(P<0.05)reduced seizures,prevented adverse cognitive behaviors,decreased the nitric oxide level,and reduced the temporal lobe Nissl chromatolysis compared with the R.vomitoria crude extract pretreatment group and the sodium valproate pretreatment groups.Conclusions Thus,R.vomitoria phenol extract showed promising results against seizures and potential for general brain protection,suggesting that the anticonvulsant property of R.vomitoria may be attributed to its phenol constituent.More studies are needed to delineate the mechanisms of its action.

关 键 词：SEIZURE Cognitive behaviour Neurochemicals Temporal lobe Sodium valproate Swiss mice 

分 类 号：R74[医药卫生—神经病学与精神病学]