动物双歧杆菌乳亚种Bi66缓解便秘及调节肠道菌群作用  

Effect of Bifidobacterium animalis subsp.lactis Bi66 on Alleviating Constipation and Regulating Gut Microbiota

作　　者：陈智仙 彭宁 张彦 CHEN Zhixian;PENG Ning;ZHANG Yan(National Key Laboratory of Agricultural Microbiology,Yichang 443003,China;The Hubei Provincial Key Laboratory of Yeast Function,Yichang 443003,China;Yichang Engineering and Technology Research Center of Nutrition and Health Food,Yichang 443003,China)

机构地区：[1]农业微生物资源发掘与利用全国重点实验室,湖北宜昌443003 [2]酵母功能湖北省重点实验室,湖北宜昌443003 [3]宜昌市营养健康食品工程技术研究中心,湖北宜昌443003

出　　处：《食品工业科技》2025年第7期308-314,共7页Science and Technology of Food Industry

基　　金：老年人群营养健康食品创制与产业化示范(应用示范类)(2023YFF1104400)。

摘　　要：目的:研究动物双歧杆菌乳亚种Bi66(Bifidobacterium animalis subsp.lactis Bi66)缓解便秘的作用,探究其作用机制,为国产菌种资源提供优质菌株。方法:将雄性Balb/c小鼠随机分为空白对照组、模型组、动物双歧杆菌乳亚种Bi66组和动物双歧杆菌乳亚种BB12组,连续给小鼠灌胃益生菌14 d后灌胃洛哌丁胺构建便秘小鼠模型,测定小鼠粪便指标、小肠推进率,测定小鼠血清胃肠调节肽,使用16S rRNA基因测序分析肠道菌群。结果:动物双歧杆菌乳亚种Bi66显著提高便秘小鼠粪便粒数、粪便湿重、粪便含水量(P<0.05),显著缩短排首粒黑便时间(P<0.05),提高小肠推进率。动物双歧杆菌乳亚种Bi66显著提高胃动素(P<0.05)、非常显著提高胃泌素水平(P<0.01)、降低血管活性肠肽、内皮素水平(P<0.05)。肠道菌群分析表明:动物双歧杆菌乳亚种Bi66提高便秘小鼠的肠道菌群丰度和多样性。Bi66降低便秘小鼠厚壁菌门,增加拟杆菌门;在属水平上显著提高另枝菌属、罗氏菌属和联合乳杆菌属丰度(P<0.05),非常显著提高经黏液真杆菌属、unclassified-Muribaculaceae和拟普雷沃氏菌属丰度(P<0.01)。结论:动物双歧杆菌乳亚种Bi66通过调节胃肠调节肽和肠道菌群缓解小鼠便秘。Objective:The objective of this study was to evaluate the efficacy of Bifidobacterium animalis subsp.lactis Bi66 in alleviating constipation in mice and exploring its mechanism,thereby providing a high-quality strain for native culture resources.Methods:Male Balb/c mice were randomly divided into the control group,the constipation model group,the Bi66 group,and the BB12 group.Probiotics were orally administered to the mice for 14 days followed by loperamide administration to induce constipation.Fecal indexes,intestinal propulsive rate,and serum gastrointestinal regulatory peptides of mice were measured,and gut microbiota was analyzed by 16S rRNA gene sequencing.Result:Bifidobacterium animalis subsp.lactis Bi66 significantly increased the number of feces particles,fecal wet weight,and fecal water content(P<0.05).It also significantly reduced the time of first black stool(P<0.05)and improved the intestinal propulsive rate in constipated mice.Moreover,Bi66 significantly improved motilin(P<0.05),gastrin(P<0.01),and decreased vasoactive intestinal peptide and endothelin levels(P<0.05).The analysis of gut microbiota revealed that the administration of Bifidobacterium animalis subsp.lactis Bi66 enhanced both the abundance and diversity of intestinal flora in constipated mice.Specifically,Bi66 exhibited a remarkable reduction in Firmicutes while promoting an increase in Bacteroidetes in constipated mice.At the genus level,the relative abundances of Alistipes,Roseburia,Ligilactobacillus,Blautia,unclassified-Muribaculaceae,and Alloprevotella showed significant increase(P<0.05,P<0.01).Conclusion:Bifidobacterium animalis subsp.lactis Bi66 alleviated constipation in mice by regulating gastrointestinal regulatory peptides and gut microbiota.

关 键 词：动物双歧杆菌乳亚种Bi66 便秘 肠道菌群 胃肠调节肽 

分 类 号：TS201.3[轻工技术与工程—食品科学]