热灭活芽孢杆菌DU-106对高脂秀丽隐杆线虫降脂作用  

Lipid-lowering Effect of Thermally Inactivated Bacillus sp.DU-106 on High-fat C.elegans

作　　者：李彦力 赵文俊 王梓宇 郭荣香 王琨 江冰雁 刘琳 陈颖仪 齐睿达 黎攀 杜冰[1] LI Yanli;ZHAO Wenjun;WANG Ziyu;GUO Rongxiang;WANG Kun;JIANG Bingyan;LIU Lin;CHEN Yingyi;QI Ruida;LI Pan;DU Bing(College of Food Science,South China Agricultural University,Guangzhou 510642,China)

机构地区：[1]华南农业大学食品学院,广东广州510642

出　　处：《食品工业科技》2025年第7期325-336,共12页Science and Technology of Food Industry

基　　金：财政部和农业农村部:国家现代农业产业技术体系资助(CARS-21)。

摘　　要：本文主要探讨热灭活芽孢杆菌DU-106(Thermally inactivated Bacillus sp.DU-106,TIDU-106)对高脂秀丽隐杆线虫降脂作用。以高糖(10 mmol/L)诱导建立高脂线虫模型,并测定不同浓度(2.5、5.0、10 mg/mL)的TIDU-106干预对高脂线虫的寿命、行动力、产卵量、活性氧水平、抗应激能力、抗氧化酶活力和脂质代谢相关基因的表达产生的影响以及高脂秀丽隐杆线虫体内脂肪沉积情况。根据野生型线虫实验结果,选取5.0、10 mg/mL浓度的TIDU-106干预基因缺陷型线虫来验证降脂效果。结果表明,经不同剂量(2.5、5.0、10 mg/mL)的TIDU-106干预后的高脂肪线虫的最大寿命显著增加(P<0.05),分别延长了35.11%、43.84%、45.63%,行动力提升,产卵量增加,活性氧水平和线粒体膜电位下降。并且提升了线虫体内超氧化物歧化酶、过氧化氢酶和谷胱甘肽过氧化物酶水平,降低了线虫体内MDA水平。线虫体内脂质代谢相关基因通路(SREBP信号通路、胰岛素/IGF-1信号通路、核受体信号通路和AMPK信号通路)的关键基因(mdt-15、sbp-1、fat-5、fat-7、daf-2、daf-16、skn-1、sod-3、sir-2.1、aak-2、nhr-49、acs-2和kat-1)受到调控。在TIDU-106干预后,对fat-5、fat-5/fat-6、fat-7和daf-16四种基因缺陷型线虫甘油三酯含量有显著降低作用(P<0.05)。对daf-2、sir-2.1和aak-2三种基因缺陷型线虫体内甘油三酯含量无明显影响(P>0.05)。综上所述,TIDU-106可通过减少脂肪沉积,增强抵抗氧化应激能力和提高抗氧化酶活性发挥其对高脂线虫的降脂作用。This study explored the lipid-lowering efficacy of thermally inactivated Bacillus sp.DU-106(TIDU-106)on Caenorhabditis elegans.A high-fat model of C.elegans was developed using a high-glucose induction method with a concentration of 10 mmol/L.To evaluate the effects of TIDU-106 on high-fat Caenorhabditis elegans,concentrations of 2.5,5.0,and 10 mg/mL were applied,assessing their impact on lifespan,locomotion,egg-laying capability,levels of reactive oxygen species(ROS),resistance to stress,activity of antioxidant enzymes,expression of genes related to lipid metabolism,and fat accumulation.Initial investigations in wild-type worms led to the selection of 5.0 mg/mL and 10 mg/mL concentrations of TIDU-106 for further examination of its lipid-lowering properties in gene-deficient worms.The results demonstrated that TIDU-106 treatment significantly increased the maximum lifespan of high-fat worms by 35.11%,43.84%,and 45.63%with varying doses(2.5,5.0,10 mg/mL)(P<0.05).These enhancements were accompanied by improved locomotion,a higher egg-laying capacity,and a decreased both in ROS levels and mitochondrial membrane potential.Additionally,treatment with TIDU-106 resulted in increased levels of superoxide dismutase,catalase,and glutathione peroxidase,coupled with a decrease in malondialdehyde levels.Notably,TIDU-106 was found to modulate key signaling pathways related to lipid-lowering,including the SREBP signaling pathway,the insulin/IGF-1 signaling pathway,the nuclear receptor signaling pathway,and the AMPK signaling pathway,as well as key genes such as mdt-15,sbp-1,fat-5,fat-7,daf-2,daf-16,skn-1,sod-3,sir-2.1,aak-2,nhr-49,acs-2,and kat-1.Specifically,after the administration of TIDU-106,there was a significant reduction in triglyceride levels in fat-5,fat-5/fat-6,fat-7,and daf-16 mutant worms(P<0.05),with daf-2,sir-2.1,and aak-2 mutants exhibiting no significant alterations(P>0.05).Above findings revealed the potential of TIDU-106 to mitigate fat deposition,boost resistance to oxidative stress,and increase antioxidant

关 键 词：芽孢杆菌DU-106 热灭活 秀丽隐杆线虫 脂代谢 抗氧化 

分 类 号：TS201.4[轻工技术与工程—食品科学]