青钱柳对2型糖尿病大鼠血糖及肠道GLP-1分泌的影响  

Effect of Cyclocarya paliurus on Glucose Metabolism and Intestinal GLP-1 Secretion in Type 2 Diabetic Rats

作　　者：邹雅倩 陈靓 慕佳 王雅靖 陈絮蒙[1] ZOU Yaqian;CHEN Liang;MU Jia;WANG Yajing;CHEN Xumeng(College of Pharmacy,Hunan University of Chinese Medicine,Changsha 410208,China)

机构地区：[1]湖南中医药大学药学院,湖南长沙410208

出　　处：《食品工业科技》2025年第7期337-345,共9页Science and Technology of Food Industry

基　　金：长沙市自然科学基金项目(kq2402185);湖南省自然科学基金项目(2024JJ8195);湖南省中医药管理局科研项目(B2024009);省级大学生创新创业计划项目(S202410541080);湖南中医药大学大学本科生科研创业基金项目(2023BKS190);湖南省药学重点学科资助。

摘　　要：目的:研究青钱柳对于2型糖尿病大鼠的降血糖作用及对肠道胰高血糖素样肽-1(glucagon-like peptide-1,GLP-1)分泌的影响,并初步探讨其作用机制。方法:高脂高糖饮食联合链脲佐菌素构建2型糖尿病大鼠模型,分别给予低剂量青钱柳(400 mg/kg)、高剂量青钱柳(800 mg/kg)以及二甲双胍(150 mg/kg),连续灌胃给药8周,每周称量大鼠体质量,记录摄食量,测定空腹血糖值。给药8周后,进行口服糖耐量试验。测定大鼠血清中糖化血红蛋白百分含量、空腹胰岛素、GLP-1、谷丙转氨酶、谷草转氨酶水平及肝脏组织中糖原水平、葡萄糖激酶(glucokinase,GK)活性;HE染色法观察大鼠肝脏病理学变化;Real-time PCR法检测结肠组织中GCG、PC1/3及肝脏组织中GK mRNA表达的相对量;免疫组化法检测结肠组织中GLP-1的蛋白表达。结果:与模型组相比,青钱柳低、高剂量组可有效控制大鼠摄食量,稳定体质量,正向调节T2DM动物基本状态;青钱柳低、高剂量组大鼠糖化血红蛋白百分含量、胰岛素抵抗指数水平均极显著低于模型组(P<0.01),青钱柳高剂量组大鼠空腹胰岛素水平极显著低于模型组(P<0.01),胰岛素敏感指数水平则显著高于模型组(P<0.05);HE染色结果表明,青钱柳可有效改善2型糖尿病引起的肝脏病理损伤;肝脏及结肠组织免疫组化及Real-time PCR结果表明,青钱柳可显著提高循环及结肠组织中GLP-1表达及分泌水平(P<0.05,P<0.01),提高肝脏GK活性及mRNA表达,恢复肝糖原水平(P<0.05,P<0.01)。结论:青钱柳可有效稳定血糖、改善胰岛素抵抗并调节GLP-1合成与分泌,对肝脏有一定保护作用,其机制涉及肝肠轴调节。Objective:This study aimed to evaluate the hypoglycemic effect of Cyclocarya paliurus on type 2 diabetic rats and its impact on glucagon-like peptide-1(GLP-1)secretion,and to preliminarily explore its mechanism.Methods:Type 2 diabetes was induced in rats using a high-fat,high-sugar diet combined with streptozotocin.Rats were then treated with low-dose(400 mg/kg)and high-dose(800 mg/kg)Cyclocarya paliurus,or metformin(150 mg/kg),via gavage for 8 weeks.Weekly assessments included body weight,food intake,and fasting glucose levels.After 8 weeks,an oral glucose tolerance test was conducted.Serum levels of glycated hemoglobin,fasting insulin,GLP-1,alanine aminotransferase,and aspartate aminotransferase were measured.Liver glycogen levels and glucokinase(GK)activity were assessed,and liver pathology was examined using HE staining.Real-time PCR analyzed mRNA expression of GCG,PC1/3 in colon tissues,and GK in liver tissues,while immunohistochemistry was used to detect GLP-1 protein expression in colon tissues.Results:Cyclocarya paliurus treatment(both low-and high-doses)effectively reduced food intake and stabilized body weight in diabetic rats,positively influencing their diabetes condition compared to the model group.Glycated hemoglobin and the insulin resistance index were significantly lower(P<0.01)in both low-and high-doses Cyclocarya paliurus-treated rats.Fasting insulin was significantly lower(P<0.01),while the insulin sensitivity index was significantly higher in the highdose group than that in the model group(P<0.05).HE staining revealed improved liver pathology in Cyclocarya paliurustreated groups.Immunohistochemistry and Real-time PCR results indicated increased GLP-1 expression and secretion in both circulating blood and colon tissues(P<0.05,P<0.01)and enhanced activity and mRNA expression of GK in liver(P<0.01).Additionally,hepatic glycogen levels were elevated(P<0.05,P<0.01).Conclusion:Cyclocarya paliurus could effectively stabilize blood glucose levels,improve insulin resistance,and regulate GLP-1 synthesi

关 键 词：青钱柳 2型糖尿病 胰高血糖素样肽-1 葡萄糖激酶 肝肠轴 

分 类 号：R151.3[医药卫生—营养与食品卫生学]