基于磁性金属有机框架纳米酶的比色适配体传感器一步法检测鱼肉中组胺  

One-Step Rapid Detection of Histamine in Fish Using a Colorimetric Aptasensor Based on Magnetic Metal Organic Framework Nanozymes

作　　者：裘奕星 刘鑫[1] 宋羽梵 丁玉庭[1,2] 徐霞 QIU Yixing;LIU Xin;SONG Yufan;DING Yuting;XU Xia(College of Food Science and Technology,Zhejiang University of Technology,Hangzhou 310014,China;Zhejiang Key Laboratory of Green,Low-carbon and Efficient Development of Marine Fishery Resources,National R&D Branch Center for Pelagic Aquatic Products Processing(Hangzhou),Hangzhou 310014,China)

机构地区：[1]浙江工业大学食品科学与工程学院,浙江杭州310014 [2]全省深蓝渔业资源绿色低碳高效开发重点实验室,国家远洋水产品加工技术研发分中心(杭州),浙江杭州310014

出　　处：《食品科学》2025年第7期318-327,共10页Food Science

基　　金：浙江省基础公益研究计划项目(LTGN23C200017);浙江省“尖兵”“领雁”研发攻关计划项目(2022C02025);2024年浙江省大学生科技创新活动计划暨新苗人才计划项目(2024R403A041);2023年浙江工业大学大学生创新创业训练计划项目(2023093)。

摘　　要：本研究以Fe3O4为核材,采用原位生长铁基金属有机框架(metal organic framework,MOF)制备磁性MOF颗粒,然后将Pt纳米颗粒掺杂在其表面制备磁性纳米酶,并将组胺适配体H1修饰到纳米酶表面得到比色探针。利用该探针与不同浓度组胺结合后,适配体构象变化所导致的纳米酶催化H_(2)O_(2)介导3,3,5,5-四甲基联苯胺氧化的显色反应差异,实现鱼肉中组胺的一步法比色传感检测。结果表明,该传感器对组胺的线性检测范围为0.1~70 μmol/L,检测限为0.943 μmol/L,鲭鱼样本的加标回收率为90.7%~100.5%。该方法线性检测范围宽、灵敏度高、特异性强,可用于鱼肉等复杂食品样品中组胺的快速检测。In this study,magnetic metal organic framework(MOF)particles were prepared using in situ grown iron-based MOF with Fe3O4 as the core,and then a magnetic nanozyme was prepared by doping the surface of the MOF particles with Pt nanoparticles,whose surface was subsequently modified with histamine aptamer H1 to obtain a colorimetric probe.After the probe was combined with different concentrations of histamine,the aptamer was conformationally changed,causing differences in the color reaction from H_(2)O_(2)-mediated tetramethylbenzidine(TMB)oxidation catalyzed by the nanozyme and thereby enabling one-step colorimetric detection of histamine in fish.The results showed that the linear detection range of the developed sensor was 0.1–70μmol/L,the detection limit was 0.943μmol/L,and the recoveries for spiked mackerel samples were 90.7%-100.5%.This method has a wide linear detection range,high sensitivity and strong specificity,and can be used for the rapid detection of histamine in fish and other complex food samples.

关 键 词：磁性金属有机框架 纳米酶 组胺 适配体传感器 比色检测 

分 类 号：TS254.7[轻工技术与工程—水产品加工及贮藏工程]