核桃油和花生油对动脉粥样硬化的改善作用  

Ameliorative effect of walnut oil and peanut oil on atherosclerosis

作　　者：胡淑娟 刘当 丁一庭 刘璇[1] 夏若寒 汪献旺 Hu Shujuan;Liu Dang;Ding Yiting;Liu Xuan;Xia Ruohan;Wang Xianwang(School of Education and Physical Education,Yangtze University,Jingzhou 423000,Hubei Province,China;Department of Medicine,Yangtze University,Jingzhou 423000,Hubei Province,China)

机构地区：[1]长江大学教育与体育学院,湖北省荆州市423000 [2]长江大学医学部,湖北省荆州市423000

出　　处：《中国组织工程研究》2025年第30期6482-6488,共7页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金项目(81974115),项目负责人:夏若寒;山南市本级科技计划项目(SNSBJKJJHXM2024023),项目负责人:汪献旺;长江大学大学生创新创业训练计划(Yz2023165),指导老师:胡淑娟。

摘　　要：背景:核因子κB/NOD样受体热蛋白结构域相关蛋白3信号的激活会导致内皮功能障碍、氧化应激等,在引发脂质代谢紊乱以及动脉硬化中起着关键作用,目前核桃油和花生油对动脉硬化大鼠骨骼肌炎症因子的影响仍不清楚。目的:探讨核桃油和花生油对动脉粥样硬化的改善效果及其作用机制。方法:40只8周龄雄性SD大鼠,适应性喂养1周后,随机分为正常对照组(n=10)和高脂组(n=30)。采用高脂饮食结合腹腔注射维生素D3建立动脉粥样硬化大鼠模型,将建模成功的大鼠随机分为模型组(n=10)、花生油组(n=8)和核桃油组(n=8),后2组连续灌胃4周花生油或核桃油[5 d/周,1.2 g/(kg·d)]。干预结束后,采用ELISA法检测大鼠血脂相关指标,苏木精-伊红染色观察大鼠主动脉形态学改变,RT-qPCR、Western blot检测骨骼肌中核因子κB、NOD样受体热蛋白结构域相关蛋白3、半胱氨酸蛋白酶1、白细胞介素1β、白细胞介素18 mRNA表达水平及核因子κB、NOD样受体热蛋白结构域相关蛋白3、白细胞介素1β蛋白表达水平,免疫荧光染色检测核因子κB和NOD样受体热蛋白结构域相关蛋白3的表达。结果与结论:①与正常对照组相比,模型组大鼠主动脉血管壁增厚,破损、脂质沉淀现象较为严重,血脂水平和动脉硬化指数显著上升(P<0.01),骨骼肌中的核因子κB、NOD样受体热蛋白结构域相关蛋白3、半胱氨酸蛋白酶1、白细胞介素1β、白细胞介素18 mRNA表达以及核因子κB、NOD样受体热蛋白结构域相关蛋白3、白细胞介素1β蛋白表达显著增加(P<0.01);②与模型组相比,花生油组和核桃油组主动脉组织易损面积明显减少,血清中总胆固醇、三酰甘油、低密度脂蛋白胆固醇水平以及动脉硬化指数显著下降(P<0.01),骨骼肌中核因子κB、NOD样受体热蛋白结构域相关蛋白3、半胱氨酸蛋白酶1、白细胞介素1β、白细胞介素18 mRNA表达以及核因子κB、BACKGROUND:Activation of nuclear factor-κB/NOD-like receptor thermal protein domain associated protein 3(NLRP3)signaling leads to endothelial dysfunction,oxidative stress,and plays a key role in the initiation of lipid metabolism disorders and arteriosclerosis.However,currenty,the effect of walnut oil and peanut oil on skeletal muscle inflammatory factors in arteriosclerotic rats remains unclear.OBJECTIVE:To discuss the effect and mechanism of walnut oil and peanut oil on atherosclerosis.METHODS:Forty 8-week-old SD male rats were randomly divided into normal control group(n=10)and high fat group(n=30)after 1 week of adaptive feeding.The atherosclerosis model was established by high-fat diet combined with vitamin D3 injection.The rats with successful modeling were randomly divided into model group(n=10),peanut oil group(n=8)and walnut oil group(n=8).The latter two groups were gavaged with peanut oil or walnut oil for 4 weeks(5 days/week,1.2 g/kg per day).After the intervention,ELISA was used to detect the related indexes of blood lipids in rats.The morphological changes of aorta were observed by hematoxylin-eosin staining.The RT-qPCR and western blot assay were used to detect nuclear factor-κB,NLRP3,Caspase-1,interleukin-1β,interleukin-18 mRNA and nuclear factor-κB,NLRP3,interleukin-1βprotein expression levels in skeletal muscle.The protein expressions of nuclear factor-κB and NLRP3 were detected by immunofluorescence immunohistochemical staining.RESULTS AND CONCLUSION:(1)Compared with the normal control group,the aortic wall of rats in the model group was thickened,the damage and lipid precipitation were more serious,the blood lipid levels and arteriosclerosis index were significantly increased(P<0.01).The mRNA levels of nuclear factor-κB,NLRP3,Caspase-1,interleukin-1β,and interleukin-18,and the protein expressions of nuclear factor-κB,NLRP3,and interleukin-1βin skeletal muscle were significantly increased(P<0.01).(2)Compared with model group,the vulnerable area of aortic tissue in peanut oil group and

关 键 词：核桃油 花生油 动脉粥样硬化 大鼠 信号通路 骨骼肌 炎症因子 工程化组织构建 

分 类 号：R459.9[医药卫生—治疗学] R318[医药卫生—临床医学] R587.1