Proteomics analysis reveals the differential protein expression of female and male adult Toxocara canis using Orbitrap Astral analyzer  

作　　者：Hui-Jie Qiu Ya-Jia Zhou Zhi-Yu Li Yi-Han Lv Xing-Quan Zhu Wen-Bin Zheng 

机构地区：[1]不详

出　　处：《Infectious Diseases of Poverty》2024年第5期103-103,共1页贫困所致传染病(英文)

摘　　要：Background Toxocara canis,the most prevalent helminth in dogs and other canines,is one of the socioeconomically important zoonotic parasites,particularly affecting pediatric and adolescent populations in impoverished communities.However,limited information is available regarding the proteomes of female and male adult T.canis.To address this knowledge gap,we performed a comprehensive proteomic analysis to identify the proteins with differential abundance(PDAs)and gender-specifically expressed proteins between the two sexes adult T.canis.Methods The comparative proteomic analysis was carried out by the Orbitrap mass spectrometry(MS)with asymmetric track lossless(Astral)analyzer.The difference analysis was conducted using t-test and the proteins verification was achieved through parallel reaction monitoring(PRM).The potential biological functions of identified adult T.canis proteins and PDAs were predicted by Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)databases.The domain,transcription factor and subcellular localization of the identified proteins and PDAs were analyzed by InterPro,AnimalTFDB 4.0 and Cell-mPLOC 2.0 databases,respectively.Results A total of 8565 somatic proteins of adult T.canis were identified.Compared to male adult,682 up-regulated PDAs and 844 down-regulated PDAs were identified in female adult with P-values<0.05 and|log2FC|>1,including 139 proteins exclusively expressed in female and 272 proteins exclusively expressed in male.The GO annotation analysis using all PDAs revealed that the main biological processes,cellular components and molecular functions corresponded to aminoglycan metabolic process,extracellular region and protein tyrosine phosphatase activity,respectively.The KEGG analysis using all PDAs showed that the pathways were mainly associated with adipocytokine signaling pathway,proximal tubule bicarbonate reclamation and PPAR signaling pathway.Conclusions This study reveals the differential protein expression between female and male adult T.canis,providing valuab

关 键 词：Toxocara canis TOXOCARIASIS ZOONOSIS Orbitrap Astral PROTEOMICS REPRODUCTION 

分 类 号：R57[医药卫生—消化系统]