Investigation of parasite genetic variation and systemic immune responses in patients presenting with different clinical presentations of cutaneous leishmaniasis caused by Leishmania aethiopica  

作　　者：Endalew Yizengaw Yegnasew Takele Susanne Franssen Bizuayehu Gashaw Mulat Yimer Emebet Adem Endalkachew Nibret Gizachew Yismaw Edward Cruz Cervera Kefale Ejigu Dessalegn Tamiru Abaineh Munshea Ingrid Müller Richard Weller James A.Cotton Pascale Kropf 

机构地区：[1]不详

出　　处：《Infectious Diseases of Poverty》2024年第5期104-105,共2页贫困所致传染病(英文)

摘　　要：Background Cutaneous leishmaniasis(CL)is a neglected tropical skin disease,caused by the protozoan parasite Leishmania.In Ethiopia,CL is mainly caused by Leishmania aethiopica and can present in different clinical forms.The aim of this study was to assess whether these different forms are associated with differences in parasite genetic and host systemic immune signatures.Methods Here we analysed the whole genome sequence data for 48 clinical parasite isolates and the systemic immune signature from a cohort of CL patients,who were recruited in Nefas Mewcha,Northern Ethiopia,from January 2019 to January 2022.Results Our results show that parasites from CL cases with different presentations in a single Ethiopian setting are from the same genetic population based on a permutation test of genome-wide similarity.Furthermore,a logistic regression test for genome wide association did not identify any individual genetic variants significantly associated with disease presentation.We also measured plasma chemokine and cytokine levels of 129 CL patients presenting with different forms of CL.None of the chemokine[eotaxin,eotaxin-3,interleukin(IL)-8,interferon(IFN)-γ-induced protein-10(IP-10),monocyte chemoattractant protein(MCP)-1,MCP-4,macrophage-derived chemokines(MDC),macrophage inflammatory protein(MIP)-1α,MIP-1βand thymus-and activation-regulated chemokine(TARC)]or cytokine(IFN-γ,IL-1β,interleukin-2,IL-4,IL-6,IL-10,IL-12p70,IL-13,tumor necrosis factor-α)levels measured were significantly different between the different clinical presentations of CL,as measured by Kruskal–Wallis test.We also compared those with healthy nonendemic controls:our results show a chemokine(IP-10,MCP-1,MCP-4,MDC,MIP-1α,MIP-1βand TARC)but not a cytokine immune signature in patients with CL as compared to healthy nonendemic controls,as measured by Mann-Whitney test.Conclusions The results of our study did not identify a systemic immune signature or parasite genetic factors associated with different clinical presentation of CL.

关 键 词：Cutaneous leishmaniasis Leishmania aethiopica Genetics CYTOKINES CHEMOKINES Plasma 

分 类 号：R73[医药卫生—肿瘤]