羧基化纤维素纳米纤维对金线鱼鱼糜冻融稳定性的影响  

Effect of Carboxylated Cellulose Nanofibers on Freeze‐Thaw Stability of Nemipterus virgatus Surimi

作　　者：米红波 郑传海 赵育茗 陈敬鑫 李学鹏 励建荣 MI Hongbo;ZHENG Chuanhai;ZHAO Yuming;CHEN Jingxin;LI Xuepeng;LI Jianrong(College of Food Science and Engineering,Bohai University,National R&D Branch Center of Surimi and Surimi Products Processing,National&Local Joint Engineering Research Center of Storage,Processing and Safety Control Technology for Fresh Agricultural and Aquatic Products,Jinzhou 121013,Liaoning,China;Collaborative Innovation Center of Seafood Deep Processing,Dalian Polytechnic University,Dalian 116034,Liaoning,China)

机构地区：[1]渤海大学食品科学与工程学院,国家鱼糜及鱼糜制品加工技术研发分中心,生鲜农产品贮藏加工及安全控制技术国家地方联合工程研究中心,辽宁锦州121013 [2]海洋食品精深加工关键技术省部共建协同创新中心,大连工业大学,辽宁大连116034

出　　处：《食品研究与开发》2025年第6期57-64,共8页Food Research and Development

基　　金：辽宁省教育厅2021年度科学研究经费面上项目(LJKZ1018)。

摘　　要：该文研究添加不同浓度的羧基化纤维素纳米纤维(0%、0.5%、1.0%、1.5%、2.0%)的金线鱼鱼糜在5次冻融循环过程中pH值、巯基含量、羰基含量、Ca^(2+) -三磷酸腺苷酶活性、硫代巴比妥酸反应物(thiobarbituricacid reactivesub-stances,TBARS)值、蛋白质二级结构和十二烷基硫酸钠-聚丙烯酰胺凝胶电泳的变化,以明确羧基化纤维素纳米纤维对金线鱼鱼糜冻融稳定性的影响。结果表明,羧基化纤维素纳米纤维可有效降低冻融过程中鱼糜蛋白的变性聚集程度,其中添加1.0%羧基化纤维素纳米纤维可抑制冻融期间总巯基、活性巯基含量和Ca^(2+) -三磷酸腺苷酶活性的下降,且能有效减缓pH值、TBARS值及羰基含量的上升,最接近商业抗冻剂对鱼糜的保护作用。同时,羧基化纤维素纳米纤维可稳定冻融过程中鱼糜肌原纤维蛋白的二级结构并抑制肌球蛋白重链的交联。因此,羧基化纤维素纳米纤维可作为新型抗冻剂用于提高金线鱼鱼糜的冻融稳定性,且1.0%羧基化纤维素纳米纤维对鱼糜肌原纤维蛋白的冻融保护效果与商业抗冻剂最接近。In order to clarify the effect of carboxylated cellulose nanofibers on the freeze-thaw stability of Nemipterus virgatus surimi,the changes in pH value,sulfhydryl content,carbonyl content,Ca^(2+) -adenosine triphos-phate ase activity,thiobarbituric acid reactive substances(TBARS),secondary structure of the protein,and sodium dodecyl sulfate-polyacrylamide gel electrophoresis(SDS-PAGE)of Nemipterus virgatus surimi with different concentrations of carboxylated cellulose nanofibers(0%,0.5%,1.0%,1.5%,and 2.0%)during five freeze-thaw cycles were investigated.The results showed that carboxylated cellulose nanofibers could effectively reduce the degree of denaturation and aggregation of protein in surimi during freeze-thaw cycles.The addition of 1.0%carboxylated cellulose nanofibers could significantly inhibit the decrease in total sulfhydryl content,active sulfhydryl content,and Ca^(2+) -adenosine triphosphate ase activity during freeze-thaw cycles and effectively slow down the increase in pH value,TBARS value,and carbonyl content,which showed the closest preservation effect of commercial antifreeze on surimi.At the same time,carboxylated cellulose nanofibers could stabilize the secondary structure of the myofibrillar protein of surimi and inhibit the crosslinking of heavy myosin chains during freeze-thaw cycles.Therefore,carboxylated cellulose nanofibers could be used as new antifreeze to improve the freeze-thaw stability of Nemipterus virgatus surimi,and the protection effect of 1.0% carboxylated cellulose nanofibers during freeze-thaw cycles on the myofibrillar protein of surimi was the closest to that of commercial antifreeze.

关 键 词：羧基化纤维素纳米纤维 鱼糜 商业抗冻剂 肌原纤维蛋白 冻融循环 

分 类 号：TQ3[化学工程]