传统发酵海产品中产香产蛋白酶菌株的筛选、鉴定及特性  

Screening,Identification and Characterization of Aroma and Protease‐producing Strains from Traditional Fermented Seafoods

作　　者：蔡浩田 陶磊 刘宇 孙迪 马庆保 余中节 姜维 CAI Haotian;TAO Lei;LIU Yu;SUN Di;MA Qingbao;YU Zhongjie;JIANG Wei(National Engineering Research Center for Marine Aquaculture,Zhejiang Ocean University,Zhoushan 316022,Zhejiang,China;College of Food and Pharmacy,Zhejiang Ocean University,Zhoushan 316022,Zhejiang,China)

机构地区：[1]浙江海洋大学国家海洋设施养殖工程技术研究中心,浙江舟山316022 [2]浙江海洋大学食品与药学学院,浙江舟山316022

出　　处：《食品研究与开发》2025年第6期145-151,共7页Food Research and Development

基　　金：国家重点研发计划项目(2023YFD2401501);浙江省基础公益研究计划项目(LTGN23C200013);定海区科技计划项目(2022C31007)。

摘　　要：从传统发酵海产品中筛选产香产蛋白酶菌株,探究其生长及产酶特性。以传统发酵的鱼露、虾酱和糟鱼酱为原料,筛选和鉴定高产蛋白酶并具备产香潜力的菌株,探究菌株生长和产酶特性。经嗅辨法和透明圈初筛,菌株生长活力、产蛋白酶活力、还原型辅酶Ⅰ-谷氨酸脱氢酶(nicotinamide adenine dinucleotide-glutamate dehydrogenase,NADH-GDH)活力和支链氨基酸转氨酶活力复筛,分离得到产蛋白酶活力和产香潜力显著高于其他菌株的菌株YL14和YL25(p<0.05)。经形态学观察和分子生物学鉴定,菌株YL14为热带芽孢杆菌(Bacillus tropics),YL25为吉氏不动杆菌(Acinetobacter guillouiae)。B.tropicus YL14和A.guillouiae YL25均在最适温度35℃、初始pH7、低盐(0%~2%)环境下生长良好。B.tropicus YL14和A.guillouiae YL25所产的蛋白酶均在温度35℃、初始pH7、NaCl含量2%时具有最高的产蛋白酶活力。This study screened out aroma and protease-producing strains from traditional fermented seafood and discussed their growth and protease-producing characteristics.With traditional fermented fish sauce,shrimp sauce and mashed fish as raw materials,strains with high protease-producing and aroma-producing potential were screened out and identified to investigate their growth and protease-producing characteristics.After preliminary screening with olfactory identification and transparent circles,the growth activity,protease-producing activity,nicotinamide adenine dinucleotide-glutamate dehydrogenase(NADH-GDH)activity,and branched-chain amino acid transaminase activity of the strains were further screened.YL14 and YL25 strains(p<0.05)were isolated,with significantly higher protease-producing activity and aroma-producing potential than others.Through morphological observation and molecular biological identification,YL14 was Bacillus tropicus and YL25 was Acinetobacter guillouiae.B.tropicus YL14 and A.guillouae YL25 grew well under an optimal temperature of 35℃,an initial pH value of 7,and a low salt environment(0%-2%).The proteases produced by B.tropicus YL14 and A.guillouae YL25 had the highest protease activity at 35℃,initial pH7,and NaCl content of 2%.

关 键 词：发酵海产品 菌株筛选 产香 产蛋白酶 生长特性 产酶特性 

分 类 号：TS2[轻工技术与工程—食品科学与工程]