Time-Dependent Transcriptional Dynamics of Contextual Fear Memory Retrieval Reveals the Function of Dipeptidyl Peptidase 9 in Reconsolidation  

作　　者：Wen-Ting Guo Wen-Xing Li Yu-Chen Liu Ya-Bo Zhao Lin Xu Qi-Xin Zhou 

机构地区：[1]Key Laboratory of Animal Models and Human Disease Mechanisms,Laboratory of Learning and Memory,Kunming Institute of Zoology,The Chinese Academy of Sciences,Kunming 650223,China [2]Kunming College of Life Science,University of Chinese Academy of Sciences,Kunming 650204,China [3]CAS Centre for Excellence in Brain Science and Intelligent Technology,Shanghai 200031,China [4]Jiangsu Key Laboratory of Neuropsychiatric Diseases,College of Pharmaceutical Sciences,Soochow University,Suzhou 215123,China

出　　处：《Neuroscience Bulletin》2025年第1期16-32,共17页神经科学通报(英文版)

基　　金：supported by the STI2030-Major Projects(2022ZD0204900);the National Natural Science Foundation of China(32071029 and 32271080);the Strategic Priority Research Program of the Chinese Academy of Sciences(XDB32020200);the Yunnan Provincial Science and Technology Department(202402AA310014).

摘　　要：Numerous studies on the formation and consolidation of memory have shown that memory processes are characterized by phase-dependent and dynamic regulation.Memory retrieval,as the only representation of memory content and an active form of memory processing that induces memory reconsolidation,has attracted increasing attention in recent years.Although the molecular mechanisms specifc to memory retrievalinduced reconsolidation have been gradually revealed,an understanding of the time-dependent regulatory mechanisms of this process is still lacking.In this study,we applied a transcriptome analysis of memory retrieval at diferent time points in the recent memory stage.Diferential expression analysis and Short Time-series Expression Miner(STEM)depicting temporal gene expression patterns indicated that most diferential gene expression occurred at 48 h,and the STEM cluster showing the greatest transcriptional upregulation at 48 h demonstrated the most significant diference.We then screened the diferentially-expressed genes associated with that met the expression patterns of those cluster-identifed genes that have been reported to be involved in learning and memory processes in addition to dipeptidyl peptidase 9(DPP9).Further quantitative polymerase chain reaction verifcation and pharmacological intervention suggested that DPP9 is involved in 48-h fear memory retrieval and viral vector-mediated overexpression of DPP9 countered the 48-h retrieval-induced attenuation of fear memory.Taken together,our fndings suggest that temporal gene expression patterns are induced by recent memory retrieval and provide hitherto undocumented evidence of the role of DPP9 in the retrieval-induced reconsolidation of fear memory.

关 键 词：Transcriptome analysis Temporal gene expression Fear memory retrieval RECONSOLIDATION DPP9 

分 类 号：R73[医药卫生—肿瘤]