IFNα2b/5-FU inhibits proliferation and cell cycle of squamous carcinoma cell line Cal27  

作　　者：Zong-Bo Wu Gong-Yue Wang Bei Wang Xi Peng Jia-Song Wang Ming-Chang Zhang Wen Geng Hua-Tao Xie 

机构地区：[1]Department of Ophthalmology,Jingshan Union Hospital,Union Hospital,Huazhong University of Science and Technology,Jingshan 431800,Hubei Province,China [2]Department of Ophthalmology,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430022,Hubei Province,China

出　　处：《International Journal of Ophthalmology(English edition)》2025年第4期565-574,共10页国际眼科杂志(英文版)

基　　金：All data and materials are available in the article upon reasonable request to the corresponding author.Foundations:Supported by the National Natural Science Foundation of China(No.82070934;No.82171025).

摘　　要：AIM:To investigate the pathological features of ocular surface squamous neoplasia(OSSN)and evaluate the synergistic therapeutic effects of interferon-α2b(IFNα2b)and 5-fluorouracil(5-FU)on cellular proliferation,migration,apoptosis,and cell cycle of human oral squamous carcinoma cell line Cal27.METHODS:Tissue specimens from OSSN were processed with hematoxylin-eosin(HE)and immunofluorescence(IF)staining to characterize pathological changes.We analyzed the expression levels of four pivotal proteins involved in 5-FU metabolism:interferon alpha receptor(IFNAR),thymidylate synthase(TS),thymidine phosphorylase(TP),and dihydropyrimidine dehydrogenase(DPD).Cal27 cell lines were treated with a spectrum of concentrations of IFNα2b and 5-FU,either in isolation or in combination.Then,cell activity was measured utilizing CCK-8 assay and dose-effect curves were calculated,while tumor cell migration was detected by cell scratch experiments.Cal27 cells were added with IFNα2b and 5-FU in a non-constant ratio drug combination design and the corresponding combination index(CI)and fraction affected(Fa)were calculated with CompuSyn software.Western blot assay was conducted to quantify the expression of TP,TS,and DPD.Cell cycle and apoptosis were measured with flow cytometry and terminal deoxynucleotidyl transferasemediated dUTP nick and labeling(TUNEL)assay.RESULTS:Treatment with both IFNα2b and 5-FU inhibited cell proliferation.Except for the lowest and highest doses of 5-FU,CI values for all other groups were below 1,suggesting a synergistic interaction.Low concentrations of IFNα2b and 5-FU both diminished the relative mobility of Cal27 cells,instead,a stronger inhibitory effect was observed when the two drugs were co-applied.The expression levels of TP and DPD in Cal27 cells were dose-dependently increased at a low concentration of IFNα2b.Low-dose IFNα2b combined with 5-FU significantly inhibited cell proliferation in G0/G1 phase compared to 5-FU monotherapy.Medium and high doses of IFNα2b and all concentrations of 5-FU c

关 键 词：ocular surface squamous neoplasia squamous carcinoma cells interferon-α2b 5-FLUOROURACIL combination index 

分 类 号：R73[医药卫生—肿瘤]