基于网络药理学技术探讨四君子汤合失笑散治疗胃癌的作用机制  

Exploration on the mechanism of the Sijunzi decoction with Shixiao San in the treatment of gastric cancer based on network pharmacology

作　　者：蓝小梅 覃素萍 李小敏 吴奕霖 刘娜 吴长亮 LAN Xiaomei

机构地区：[1]广西中医药大学,广西南宁530200 [2]广西中医药大学附属广西国际壮医医院,广西南宁530201

出　　处：《中医临床研究》2025年第2期37-44,共8页Clinical Journal Of Chinese Medicine

基　　金：广西医学遗传与基金组学研究重点实验室2022年开放课题(GXKMGG202202);广西壮族自治区卫生和计划生育委员会自筹经费科研课题(Z20181004);2023年广西中医药多学科交叉创新团队(GZKJ2311)。

摘　　要：目的:基于网络药理学方法探讨四君子汤联合失笑散治疗胃癌的作用机制,并进行分子对接验证。方法:通过中药系统药理学数据库与分析平台(TCMSP)和中药分子机理的生物信息学分析工具(BATMAN–TCM)检索四君子汤和失笑散的活性成分及其靶点;通过OMIM、GeneCards、DrugBank、TTD数据库检索胃癌相关基因;应用韦恩在线绘制平台获取疾病和中药相互作用交集靶点。应用CytoScape 3.7.1软件构建“中药–成分–交集靶点”网络图。将疾病和中药相互作用交集靶点通过STRING 12.0数据库进行相互作用分析,构建蛋白质–蛋白质相互作用网络图,获取核心靶点。通过微生信在线平台进行基因本体论(GO)和京都基因与基因组百科全书(KEGG)通路富集分析。通过分子对接验证核心成分与靶点的结合可能性。结果:筛选得到四君子汤联合失笑散有效成分126个,与胃癌对应作用靶点161个。获得GO富集分析条目892个(P<0.05),其中生物过程条目698个,分子功能条目126个,细胞组分条目68个。KEGG通路富集筛选得到156条信号通路(P<0.05),主要涉及磷脂酰肌醇3激酶–蛋白激酶B(Phosphoinositide 3–Kinase–Akt,PI3K–Akt)信号通路、丝裂原活化蛋白激酶(Mitogen–Activated Protein Kinase,MAPK)信号通路、癌症通路等。分子对接验证结合性好。结论:四君子汤联合失笑散治疗胃癌具有“多成分–多靶点–多途径”的特点与优势,可为四君子汤联合失笑散治疗胃癌的物质基础与分子机制提供理论依据。Objective:To explore the mechanism of the Sijunzi decoction(四君子汤)with Shixiao San(失笑散)in the treatment of gastric cancer based on network pharmacology,and to verify the molecular docking.Methods:Through TCMSP and BATMAN-TCM,the active components of the Sijunzi decoction and Shixiao San and their related targets were collected and screened.The gastric cancer-related genes were retrieved from OMIM,GeneCards,DrugBank and TTD databases.Venn online diagram platform was used to obtain the intersection targets of disease and traditional Chinese medicine.Cytoscape 3.7.1 software was used to construct the network diagram of Chinese medicine-component-intersection targets.The interaction targets of disease and traditional Chinese medicine were analyzed by STRING 12.0 database,and the protein-protein interaction network diagram was constructed to obtain the core targets.GO and KEGG pathway enrichment analysis were performed through online platform WeChat.Results:A total of 126 effective components of the Sijunzi decoction and Shixiao San were screened,and 161 targets corresponding to gastric cancer were obtained.A total of 892 GO enrichment analysis items were obtained(P<0.05),including 698 biological process items,with 126 molecular function items,and 68 cell component items.KEGG pathway enrichment screening obtained 156 signaling pathways(P<0.05),mainly involving PI3K-Akt signaling pathway,MAPK signaling pathway,cancer pathway,etc.Molecular docking verified good binding.Conclusion:The Sijunzi decoction with Shixiao San has the characteristics and advantages of“multi-component,multi-target,multipathway”in the treatment of gastric cancer,which provides a theoretical basis for studying the material basis and molecular mechanism of the Sijunzi decoction with Shixiao San in the treatment of gastric cancer.

关 键 词：四君子汤 失笑散 胃癌 网络药理学 分子对接 

分 类 号：R735.2[医药卫生—肿瘤]