贵州红酸汤对高脂饮食大鼠脂质代谢的影响  

Effect of Guizhou red sour soup on lipid metabolism in rats fed a high-fat diet

作　　者：胡诗瑶 袁钦 郑远翠 王惠群[1] HU Shiyao;YUAN Qin;ZHENG Yuancui;WANG Huiqun(School of Public Health and Health,Guizhou Medical University,Guiyang 561113,China;The Key Laboratory ofEnvironmental Pollution and Disease Control,Ministry of Education,Guizhou Medical University,Guiyang 561113,China)

机构地区：[1]贵州医科大学公共卫生与健康学院,贵州贵阳561113 [2]贵州医科大学环境污染与疾病监控教育部重点实验室,贵州贵阳561113

出　　处：《中国酿造》2025年第3期100-106,共7页China Brewing

基　　金：贵州省区域内一流学科建设项目-公共卫生与预防医学(黔教科研发[2017]85号)。

摘　　要：为探究贵州红酸汤对高脂饮食大鼠脂质代谢的影响及其机制,采用无特定病原体(SPF)级SD雄性大鼠作为实验对象,随机分为三个组并持续干预12周后,称取大鼠体质量及肝脏质量,计算体质指数(BMI)和肝脏指数(LI),检测血清脂质水平,观察肝脏组织病理学变化情况,测定肝脏中白细胞分化抗原36(CD36)、脂肪酸转运蛋白4(FATP4)、过氧化物酶体增殖物激活受体α(PPARα)、肉碱棕榈酰转移酶1(CPT1)的表达水平。结果表明,贵州红酸汤能降低高脂饮食大鼠的体质量、体质指数(BMI)和肝脏指数(LI),降低其血清中甘油三酯(TG)、总胆固醇(TC)、低密度脂蛋白胆固醇(LDL-C)的水平,提高高密度脂蛋白胆固醇(HDL-C)水平,同时贵州红酸汤可抑制高脂饮食大鼠肝脏中FATP4、CD36的表达,促进PPARα、CPT1的表达。结果显示,贵州红酸汤可以预防高脂饮食大鼠肥胖的发生,改善血脂水平及肝脏脂肪变性,其作用机制可能是其抑制其肝细胞脂肪酸摄取和转运,促进其肝细胞中的脂肪酸β氧化,从而改善大鼠机体中的脂质代谢紊乱。In order to explore the effect of Guizhou red sour soup on lipid metabolism in high-fat diet rats and its mechanism,using specific pathogen-free(SPF)SD male rats as experimental subjects and randomly divided into three groups for 12 weeks of continuous intervention.The body and liver mass of the rats were weighed,the body mass index(BMI)and liver index(LI)were calculated,the serum lipid level was detected,and the histopathological changes of the liver were observed.The expression levels of leukocyte differentiation antigen 36(CD36),fatty acid transporter 4(FATP4),peroxisome proliferator-activated receptorα(PPARα)and carnitine palmitoyl transferase 1(CPT1)in liver were determined.The results showed that Guizhou red sour soup could reduce the body mass,body mass index and liver index of high-fat diet rats,reduce the levels of triglyceride(TG),total cholesterol(TC)and low-density lipoprotein cholesterol(LDL-C)in serum,and increase the level of high-density lipoprotein cholesterol(HDL-C).At the same time,Guizhou red sour soup could inhibit the expression of FATP4 and CD36 in the liver of high-fat diet rats,and promote the expression of PPARαand CPT1.The results showed that Guizhou red sour soup could prevent the occurrence of obesity in rats with high fat diet,improve blood lipid level and liver steatosis.The mechanism of its action might be that it inhibited fatty acid uptake and transport in hepatocytes and promoted theβ-oxidation of fatty acid in hepatocytes,thus improving the disorder of lipid metabolism in rats.

关 键 词：贵州红酸汤 高脂饮食 脂质代谢 作用机制 大鼠 

分 类 号：TS255.3[轻工技术与工程—农产品加工及贮藏工程]