景宁木兰花蕾挥发性化学成分的GC-MS分析  

GC-MS Analysis of Volatile Chemical Components in Magnolia sinostellata Flower Buds

作　　者：吴伟建 陈武 仇建习 石从广[4] 李凯 蒋明[6] 王军峰 WU Weijian;CHEN Wu;QIU Jianxi;SHI Congguang;LI Kai;JIANG Ming;WANG Junfeng(Songyang Natural Resources and Planning Bureau of Zhejiang,Songyang 323400,Zhejiang,China;Lishui Baiyun Ecological Forest Farm,Lishui 323000,zhejiang,china;Lishui Liandu Ecological Forestry Development Center,Lishui 323000,Zhejiang,China;Zhejiang Academy of Forestry,Hangzhou 310023,Zhejiang,China;Lishui Liandu Laozhu Forestry workstation,Lishui 323000,Zhejiang,China;College of Life Sciences,Taizhou University,Jiaojiang 318000,Zhejiang,China;Scientific Research Management Center,East China Medicinal Botanical Garden,Lishui 323000,Zhejiang,China)

机构地区：[1]松阳县自然资源和规划局,浙江松阳323400 [2]丽水市白云山生态林场,浙江丽水323000 [3]丽水市莲都区生态林业中心,浙江莲都311300 [4]浙江省林业科学研究院,浙江杭州310023 [5]丽水市莲都区老竹林业工作中心站,浙江莲都311300 [6]台州学院生命科学学院,浙江台州318000 [7]华东药用植物园科研管理中心,浙江丽水323000

出　　处：《浙江林业科技》2025年第1期53-58,共6页Journal of Zhejiang Forestry Science and Technology

摘　　要：为探究景宁木兰M.sinostellata不同大小花蕾中的挥发性化学成分,以阴干的Ⅰ~Ⅴ级花蕾为材料,利用固相微萃取和气相色谱质谱联用检测化学成分。结果表明:景宁木兰花蕾中总共检测到醇类、芳香烃类、醛类、萜烯类、酮类和酯类6类、48种挥发性物质,其中萜烯类最多,为37种。其次为醇类和芳香烃类物质,分别为5种和3种;酮类、酯类和醛类物质最少,均为1种。不同等级花蕾的质谱峰数量差异较小,其中Ⅱ级最多,为32个,其次为Ⅳ级和Ⅴ级,均为31个,Ⅰ级和Ⅲ级最少,为30个。不同等级花蕾挥发性化学成分种类数量大致相近,但所含种类却有一定的差异,主要表现为不同等级花蕾的共有物质有17种,特有物质2~3种。桉叶油醇在不同等级花蕾中的相对含量均为最高,莰烯在Ⅰ~Ⅳ级花蕾中的相对含量为第二高,而Ⅴ级花蕾中的桧烯相对含量为第二高。针对不同等级花蕾间挥发性化学成分的研究,可为后续景宁木兰的保护及利用提供科学依据。To investigate the volatile chemical components in flower buds of Magnolia sinostellata at different developmental stages,shade-dried flower buds of Grades Ⅰ to Ⅴ were analyzed using solid-phase microextraction(SPME)coupled with gas chromatography-mass spectrometry(GCMS).The results showed that a total of 48 volatile compounds,categorized into six classes:alcohols,aromatic hydrocarbons,aldehydes,terpenes,ketones,and esters were detected in the flower buds.Terpenes were the most abundant class,comprising 37 compounds,followed by alcohols(5 compounds)and aromatic hydrocarbons(3 compounds).The least abundant classes were ketones,esters,and aldehydes,each containing only 1 compound.The number of mass spectral peaks varied slightly among different grades:Grade Ⅱ exhibited the highest count(32 peaks),followed by Grades Ⅳ and Ⅴ(31 peaks each),while Grades Ⅰ and Ⅲ had the fewest(30 peaks each).Although the total number of volatile chemical component types was similar across grades,their specific compositions differed.There were 17 common compounds shared and 2-3 unique compounds among all grades.Eucalyptol consistently showed the highest relative content across all grades.Camphene ranked second in Grades Ⅰ-Ⅳ,while sabinene became the second most abundant compound in Grade V.This study on the volatile chemical components of M.sinostellata flower buds at different developmental stages provides a scientific basis for their conservation and utilization.

关 键 词：景宁木兰 花蕾 挥发成分 固相微萃取 气相色谱质谱联用 

分 类 号：S685.15[农业科学—观赏园艺]