结肠腺癌原发灶和淋巴结转移灶中P-gp、EGFR、VEGF的表达对比  

The Expression of P-gp,EGFR and VEGF between Primary Foci and Paired Lymphatic Metastasis in Adenocarcinoma of Colon

作　　者：唐道爱 李黎 马彬丹 陈宝健 TANG Daoai;LI Li;MA Bindan;CHENG Baojian(Department of Pathology,Mengzi People's Hospital,Mengzi,Yunnan 661100)

机构地区：[1]云南省蒙自市人民医院病理科,云南蒙自661100

出　　处：《智慧健康》2025年第2期26-29,共4页Smart Healthcare

摘　　要：目的比较结肠腺癌原发灶和淋巴结转移灶中P-糖蛋白(P-gp)、表皮生长因子受体(EGFR)、血管内皮生长因子(VEGF)的表达及凋亡增殖比的差异及意义。方法选取2018年1月—2022年8月本院病理科结肠腺癌标本41例,每例包括结肠腺癌原发灶和淋巴结转移灶石蜡标本(共82个标本),用免疫组织化学染色方法检测P-gp、EGFR、VEGF的表达及Ki-67指数;用实时荧光PCR半定量法检测MDR1、EGFR、VEGF基因的相对mRNA含量;用TUNEL检测试剂盒检测凋亡指数。结果P-gp、EGFR、VEGF在结肠腺癌原发灶和淋巴结转移灶内均高表达,差异无统计学意义(P>0.05);MDR1、EGFR、VEGF基因的相对mRNA含量在原发灶和淋巴结转移灶中的差异无统计学意义(P>0.05);原发灶和淋巴结转移灶中癌组织增殖指数的差异无统计学意义(P>0.05),凋亡指数和凋亡增殖比(AI/PI)在原发灶高于淋巴结转移灶(P<0.05)。结论(1)P-gp/MDR1、EGFR、VEGF在结肠腺癌原发灶和淋巴结转移灶中的表达没有显著差异;(2)免疫组化和实时荧光定量PCR检测P-gp/MDR1、EGFR、VEGF在结肠腺癌原发灶和淋巴结转移灶中的表达对结肠腺癌淋巴结转移的靶向用药没有指导意义;(3)结肠腺癌淋巴结转移灶较原发灶内癌细胞凋亡受抑制。Objective To compare the expression differences of P-gp、EGFR、VEGF and the AI/PI between primary foci and paired lymphatic metastasis in adenocarcinoma of colon.Methods 82 paraffin specimens including primary foci and paired lymphatic metastasis of adenocarcinoma of colon were collected from the pathologic department in Mengzi People’s Hospital.Immunohistochemical staining method is used to detect the expression of P-gp、EGFR、VEGF and the index of proliferation in all specimens;Real-time quantitative PCR is used to detect the mRNA relative content of MDR1、EGFR、VEGF in all specimens;TUNEL kit is used to detect the index of apoptosis.Results Immunohistochemical staining showed the high expression of P-gp、EGFR、VEGF both in primary foci and paired lymphatic metastasis,but is not statistically significant(P>0.05),neither the relative content mRNA of MDR1、EGFR、VEGF.However,the AI and AI/PI are higher in primary foci than in lymphatic metastasis,demonstrate statistically significant(P<0.05).Conclusion(1)There is no statistical significance of expression differences about P-gp/MDR1、EGFR、VEGF between primary foci and lymphatic metastasis in adenocarcinoma of colon.(2)Detecting the protein expression or mRNA relative content of P-gp/MDR1、EGFR、VEGF in adenocarcinoma of colon is ineffective for guiding the targeted therapy in lymphatic metastasis.(3)Apoptosis is suppressed in lymphatic metastasis compared to the primary foci of adenocarcinoma of colon.

关 键 词：结肠腺癌 P-GP EGFR VEGF TUNEL 

分 类 号：R73[医药卫生—肿瘤]