心脏骤停/心肺复苏模型构建时间对大鼠心脏损伤的比较  

Comparison of Cardiac Injury in a Rat Cardiac Arrest/Cardiopulmonary Resuscitation Model with Different Arrest Durations

作　　者：吴倩倩 何永璟 黄凡 王慧慧 杜子龙 谢露 WU Qian-qian;HE Yong-jing;HUANG Fan;WANG Hui-Hui;DU Zi-long;XIE Lu(Department of Physiology,College of Basic Medicine,Guangxi Medical University,Nanning,530022;The Fourth People's Hosipital of Yongzhou,Hunan Province)

机构地区：[1]广西医科大学基础医学院生理学教研室,530022 [2]湖南省永州市第四人民医院

出　　处：《岭南急诊医学杂志》2025年第1期1-3,7,共4页Lingnan Journal of Emergency Medicine

基　　金：国家自然科学基金资助项目(82160372)。

摘　　要：目的:比较心脏骤停/心肺复苏(CA/CPR)模型中不同CA时间对大鼠生存影响及心脏的损伤程度,为抗CA/CPR后心功能损伤研究模型提供基础依据。方法:40只雄性SD大鼠随机分成4组(每组n=10):假手术组(sham组)、CA7分钟组(7 min组)、CA 8分钟组(8 min组)、CA 9分钟组(9 min组)。sham组仅进行手术操作,其余组采用经食道右心室电刺激制备CA/CPR模型,观察CPR 24 h后自主循环恢复(ROSC)情况、存活情况,经心脏彩超检测心功能,经心肌切片观察病理变化。结果:随着CA时间逐步增加,大鼠ROSC情况逐渐下降,存活数量逐渐减少,心脏病理结构和功能损伤逐渐加重。与7 min组的和8 min组相比,9 min组的ROSC时间显著延长[(106±10.03)s,(126.67±7.60)s vs (158±15.16)s,P<0.05]。与7 min组的ROSC数量及存活24 h的数量比,8 min组减少无显著差异,9 min组均明显减少(10/10 vs 6/10,10/10 vs 5/10,P<0.05)。与sham组、7 min组和8 min组比,9 min组的心功能损伤加重,具体表现为左心室射血分数(LVEF)[(82.25±1.71)%,(78.25±2.5)%,(73.50±2.65)%vs(53.50±2.08)%,P<0.05]、左心室短轴缩短率(LVFS)[(45.75±2.22)%,(41.75±2.50)%,(37.75±2.22)%vs (23.50±1.29)%,P<0.05]和心输出量(CO)[(103.95±2.60)ml/min,(98.76±1.05)ml/min,(79.38±2.40)ml/min vs(57.39±2.40)ml/min,P<0.05]均显著下降。结论:不同CA时间后CPR均可对大鼠心脏造成损伤,但9 min CA造成显著的心脏结构和功能损伤,适用于CA/CPR后针对心功能的实验研究。Objective:To compare the effects of different cardiac arrest(CA)durations on survival and cardiac injury in a rat model of cardiac arrest/cardiopulmonary resuscitation(CA/CPR),and provide a foundational model for research on post‐CA/CPR cardiac dysfunction.Methods:Forty male Sprague‐Dawley rats were randomly assigned to four groups(n=10 per group):Sham group(sham),7 minutes CA group(7 min),8 minutes CA group(8 min),and 9 minutes CA group(9 min).The sham group underwent surgical procedures only,while the other groups underwent CA/CPR model induction via transesophageal right ventricular electrical stimulation.The return of spontaneous circulation(ROSC)and survival rates were monitored 24 hours post‐CPR.Cardiac function was assessed via echocardiography.Myocardial tissue was analyzed for pathological changes.Results:Prolonged CA duration progressively reduced ROSC rates,survival rates,and worsened cardiac structural and functional damage.Compared to the 7 min and 8min groups,the 9 min group exhibited significantly prolonged ROSC time[(106±10.03)s,(126.67±7.60)s vs.(158±15.16)s,P<0.05].While ROSC and 24‐hour survival rates in the 8 min group showed no significant difference compared to the 7 min group,the 9 min group demonstrated markedly reduced rates(10/10 vs.6/10,10/10 vs.5/10,P<0.05).Cardiac function in the 9 min group deteriorated significantly compared to the sham,7 min,and 8min groups,evidenced by decreased left ventricular ejection fraction(LVEF)[(82.25±1.71)%,(78.25±2.5)%,(73.50±2.65)%vs.(53.50±2.08)%,P<0.05],left ventricular fractional shortening(LVFS)[(45.75±2.22)%,(41.75±2.50)%,(37.75±2.22)%vs.(23.50±1.29)%,P<0.05],and cardiac output(CO)[(103.95±2.60)ml/min(98.76±1.05)ml/min,(79.38±2.40)ml/min vs.(57.39±2.40)ml/min,P<0.05].Conclusion:Different durations of CA followed by CPR result in significant cardiac injury in rats,with the 9‐minute CA causing severe structural and functional damage.This model is suitable for studying myocardial dysfunction after CA/CPR.

关 键 词：心脏骤停 心肺复苏 心脏损伤 

分 类 号：R-332[医药卫生] R541.78