响应面法优化马鲛鱼皮抗冻多肽的酶解工艺  

Optimization of the Enzymatic Hydrolysis Process of Antifreeze Polypeptides from Mackerel Skin by the Response Surface Methodology

作　　者：洪燕婷 颜阿娜 HONG Yanting;YAN Ana(Liming Vocational University,Quanzhou 362000,China)

机构地区：[1]黎明职业大学,福建泉州362000

出　　处：《辽宁农业职业技术学院学报》2025年第2期6-10,29,共6页Journal of Liaoning Agricultural Technical College

基　　金：2022年度福建省中青年教师教育科研项目(科技类)(JAT220703);2024年福建省高等学校应用技术工程中心项目“海洋生物质资源开发”。

摘　　要：以马鲛鱼皮为主要原料提取明胶,后采用生物酶解法制备抗冻多肽,并将抗冻多肽添加到过氧化氢酶中做低温保护试验,以过氧化氢酶残余活力大小代表抗冻活性,探索酶解影响因素,并采用响应面法优化酶解工艺条件。结果显示:在试验范围内,酸性蛋白酶效果最佳,其酶解工艺最优条件为酶解温度45℃、酶解pH6.9、酶解时间1.6h、酶添加量7500U/g,添加到过氧化氢酶中经冻融后酶残余活力为82.75%,显著高于空白和牛血清蛋白对照组,此条件下制备的马鲛鱼皮多肽的抗冻活性良好。Gelatin was extracted from the mackerel skin,and then antifreeze polypeptides were prepared by enzyme hydroly⁃sis.The antifreeze polypeptides were added to catalase for low temperature protection test.The residual activity of catalase was used to represent the antifreeze activity.The influencing factors of enzymatic hydrolysis were explored,and the enzymatic hydrolysis condi⁃tions were optimized by the response surface methodology.The results showed that the acid protease had the best effect in the experi⁃mental range.The optimal conditions for the enzymatic hydrolysis process were as follows:the enzymatic hydrolysis temperature was 45℃,the enzymatic hydrolysis pH was 6.9,the enzymatic hydrolysis time was 1.6 h,and the enzyme addition amount was 7500 U/g.The residual activity of the enzyme after freezing and thawing was 82.75%,which was significantly higher than that of the blank treat⁃ment group and bovine serum protein treatment group.The antifreeze activity of mackerel skin polypeptides prepared under this condi⁃tion was good.

关 键 词：抗冻多肽 马鲛鱼皮 过氧化氢酶 酶解工艺 

分 类 号：TS201.2[轻工技术与工程—食品科学]