A comprehensive review on microbial hyaluronan-degrading enzymes:from virulence factors to biotechnological tools  

作　　者：Jia-Yu Jiang Dai Xue Jin-Song Gong Qin-Xin Zheng Yue-Sheng Zhang Chang Su Zheng-Hong Xu Jin-Song Shi 

机构地区：[1]Key Laboratory of Carbohydrate Chemistry and Biotechnology of Ministry of Education,School of Life Sciences and Health Engineering,Jiangnan University,Lihu Avenue No.1800,Wuxi 214122,People’s Republic of China [2]The Novo Nordisk Foundation Center for Biosustainability,Technical University of Denmark,Kemitorvet 220,2800 Kgs,Lyngby,Denmark [3]School of Biotechnology,Jiangnan University,Wuxi 214122,People’s Republic of China [4]College of Biomass Science and Engineering,Sichuan University,Chengdu 610065,People’s Republic of China [5]Affiliated Children’s Hospital of Jiangnan University,Wuxi 214023,People’s Republic of China

出　　处：《Bioresources and Bioprocessing》2024年第1期1492-1514,共23页生物资源与生物加工(英文)

基　　金：financially supported by the National Key Research and Development Program of China(No.2021YFC2100900);the National Natural Science Foundation of China(No.32171261);the Taishan Industry Leading Talent of Shandong Province(No.tscx202211017);the Postgraduate Research&Practice Innovation Program of Jiangsu Province(No.KYCX23_2575).

摘　　要：Hyaluronan(HA),a natural high molecular weight polysaccharide,has extensive applications in cosmetology and medical treatment.Hyaluronan-degrading enzymes(Hyals)act as molecular scissors that cleave HA by breaking the glucosidic linkage.Hyals are present in diverse organisms,including vertebrates,invertebrates and microorganisms,and play momentous roles in biological processes.In recent years,microbial Hyals(mHyals)have gained considerable attention for their exceptional performance in the production and processing of HA.Moreover,the applications of mHyals have been greatly extended to various biomedical fields.To explore the potential applications of mHyals,a thorough comprehension is imperative.In this context,this review systematically summarizes the sources,structures,mechanisms and enzymatic properties of mHyals and discusses their biological functions in host invasion,disease development,and regulation of intestinal flora.Furthermore,versatile applications inspired by their biological functions in medicine development,molecular biology,and industrial biotechnology are comprehensively reviewed.Finally,prospects are presented to emphasize the importance of exploration,expression and characterization of mHyals and the necessity of excavating their potential in biotechnological fields.

关 键 词：Hyaluronan-degrading enzymes Hyaluronan lyase HYALURONIDASE Host invasion Disease development ANTI-BACTERIA Cancer therapy Hyaluronan degradation Hyaluronan detection 

分 类 号：R73[医药卫生—肿瘤]