苹果多酚提取物对高脂诱导肝脏SIRT1杂合突变C57BL/6小鼠肝脏脂质组学的影响  

EFFECTS OF APPLE POLYPHENOLS EXTRACT ON LIVER LIPIDOMICS IN HEPATIC SIRT1 HETEROZYGOUS MUTANT C57BL/6 MICE FED A HIGH-FAT DIET

作　　者：马婕妤 殷焱 栾文雪 尚思源 李新莉[1] MA Jie-yu;YIN Yan;LUAN Wen-xue;SHANG Si-yuan;LI Xin-li(Department of Nutrition and Food Hygiene,School of Public Health,Medical College of Soochow University,Suzhou 215123,China)

机构地区：[1]苏州大学苏州医学院公共卫生学院营养与食品卫生学系,苏州215123

出　　处：《营养学报》2024年第6期606-611,617,共7页Acta Nutrimenta Sinica

基　　金：国家自然科学基金(No.81973023)

摘　　要：目的 探讨苹果多酚提取物(APE)对高脂饮食诱导肝脏SIRT1杂合突变(SIRT1^(+/-))C57BL/6小鼠肝脏脂质组学的影响。方法 C57BL/6雄鼠与雌鼠随机分为对照组(WT+HFD),肝脏SIRT1杂合突变组(SIRT1^(+/-)+HFD),肝脏SIRT1杂合突变APE干预组(SIRT1^(+/-)+HAP),三组均给予高脂饲料喂养8w,APE干预组同时给予500 mg/(kg·bw·d)的APE溶液灌胃,每周记录小鼠体重与摄食量变化。采用液相色谱-质谱联用(LC-MS)法检测小鼠肝脏中差异表达的脂质代谢物。结果 与WT+HFD组相比,SIRT1^(+/-)+HFD组中有12种TG类的脂质水平明显升高,而APE干预后下降;除TG 52:4-18:2外,雌性SIRT1^(+/-)小鼠所有确定的TG种类的含量都明显高于雄性SIRT1^(+/-)小鼠(P<0.05),但野生型小鼠之间的脂质特征并无统计学差异。结论 APE干预通过影响脂质代谢组学成分改善高脂诱导的、肝脏SIRT1杂合突变小鼠的肝脏总脂质的变化,且APE干预对SIRT1^(+/-)雌性小鼠的改善作用优于雄性小鼠。Objective To investigate the effects of apple polyphenol extract(APE)on liver lipidomics in high-fat diet(HFD)fed C57BL/6 mice with hepatic SIRT1 heterozygous mutation(SIRT1^(+/-)).Methods C57BL/6 male and female mice were randomly divided into control group(WT+HFD),SIRT1 heterozygous mutation group(SIRT1^(+/-)+HFD),and SIRT1 heterozygous mutation with APE intervention group(SIRT1^(+/-)+HAP).All mice were fed HFD for 8 consecutive weeks,and mice in SIRT1^(+/-)+HAP group were gavaged with 500 mg/(kg·bw·d)APE solution.Body weight and food intake were recorded every week.The hepatic lipid metabolites were detected by a liquid chromatograph-mass spectrometer(LC-MS).Results Significantly increased contents of 12 TG species in the SIRT1^(+/-)+HFD group were observed compared with the WT+HFD group,which were decreased after APE intervention.Except TG 52:4-18:2,female SIRT1^(+/-)mice had significantly higher levels of all identified TG species than male SIRT1^(+/-)mice(P<0.05),but there was no significant difference in lipid profiles between the WT groups.Conclusion APE intervention improves hepatic lipids in HFD exposed SIRT1^(+/-)mice by regulating lipid metabolomics,and the ameliorated effects of APE on hepatic lipids are better in SIRT1^(+/-)female mice than in male mice.

关 键 词：苹果多酚提取物 高脂饮食 肝脏脂质组学 SIRT1^(+/-)小鼠 

分 类 号：R151.2[医药卫生—营养与食品卫生学]