Regional diferences of the sclera in the ocular hypertensive rat model induced by circumlimbal suture  

作　　者：Mingfang Xia Endong Zhang Fei Yao Zhaohua Xia Mingmin Zhou Xufang Ran Xiaobo Xia 

机构地区：[1]Eye Center of Xiangya Hospital,Central South University,Changsha 410008,Hunan,China [2]Hunan Key Laboratory of Ophthalmology,Changsha 410008,Hunan,China [3]National Clinical Research Center for Geriatric Disorders,Xiangya Hospital,Central South University,Changsha 410008,Hunan,China [4]Department of Ophthalmology,People’s Hospital of Ningxia Hui Autonomous Region,Yinchuan 750004,Ningxia,China

出　　处：《Eye and Vision》2025年第1期1-12,共12页眼视光学杂志(英文)

基　　金：funded by the National Key Research and Development Program of China(Grant No.2020YFC2008205);the National Natural Science Foundation of China(Grant Nos.82171058,81974134);Key R&D Plan of Hunan Province of China(Grant No.2020SK2076).

摘　　要：Purpose:To describe the regional diferences of the sclera in ocular hypertension(OHT)models with the inappropriate extension of the ocular axis.Methods:To discover the regional diferences of the sclera at the early stage,OHT models were established using circumlimbal suture(CS)or sclerosant injection(SI).Axial length(AL)was measured by ultrasound and magnetic resonance imaging.The glaucoma-associated distinction was determined by intraocular pressure(IOP)and retrograde tracing of retinal ganglion cells(RGCs).The central thickness of the ganglion cell complex(GCC)was measured by optical coherence tomography.RGCs and collagen fbrils were detected using a transmission electron microscope,furthermore,anti-alpha smooth muscle actin(αSMA)was determined in the early stage after the operation.Results:Compared with the control group,the eyes in OHT models showed an increased IOP(P<0.001 in the CS group,P=0.001 in the SI group),growing AL(P=0.026 in the CS group,P=0.043 in the SI group),reduction of central RGCs(P<0.001 in the CS group,P=0.017 in the SI group),thinning central GCC(P<0.001 in the CS group),and a distinctive expression ofαSMA in the central sclera in the early 4-week stage after the operation(P=0.002 in the CS group).Compared with the SI group,the eye in the CS group showed a signifcantly increased AL(7.1±0.4 mm,P=0.031),reduction of central RGCs(2121.1±87.2 cells/mm2,P=0.001),thinning central GCC(71.4±0.8 pixels,P=0.015),and a distinctive expression ofαSMA(P=0.005).Additionally,ultrastructural changes in RGCs,scleral collagen fbers,and collagen crimp were observed in the diferent regions.Increased collagen volume fraction in the posterior segment of the eyeball wall(30.2±3.1%,P=0.022)was observed by MASSON staining in the CS group.Conclusion:Regional diferences of the sclera in the ocular hypertensive rat model induced by CS may provide a reference for further treatment of scleral-related eye disorders.

关 键 词：SCLERA Axial length GLAUCOMA Retinal ganglion cells Animal models 

分 类 号：R73[医药卫生—肿瘤]