Probiotic potential of lactic acid bacteria isolated from yaks  

作　　者：Yuanyuan He Feiran Li Mengen Xu Chao Jin Yu Zhang Shah Nawaz Muhammad Fakhar-e-Alam Kulyar Mudassar Iqbal Zhen Qin Jiakui Li 

机构地区：[1]College of Veterinary Medicine,Huazhong Agricultural University,Wuhan 430070,PR China [2]College of Animals Husbandry and Veterinary Medicine,Tibet Agricultural and Animal Husbandry University,Linzhi,Tibet 860000,PR China [3]Xuanen Animal Husbandry and Veterinary Technical Service Center,Xuanen 445500,PR China

出　　处：《Animal Diseases》2025年第1期73-84,共12页动物疾病(英文)

基　　金：supported by the Key Project of Tibet Autonomous Region(XZ202001ZY0044N;XZ202101ZD0002N-05).

摘　　要：The prevalence of bacterial digestive diseases in plateau animals has caused considerable losses to the Tibetan live‑stock industry.Therefore,this study aimed to isolate safe lactic acid bacteria(LAB)with beneficial probiotic proper‑ties to protect yaks from intestinal diseases.After 16S rDNA matching,four strains of Lactobacillus fermentum(A4),Pediococcus pentosaceus(A3.4 and A1.2),and Pediococcus acidilactici(B1.9)were isolated from the intestinal tissues and content of healthy yaks.The results indicated that A4 was more tolerant to bile salt(0.3%),while A3.4 had better stability in an acidic(pH=3.0)environment.The results of the antibacterial activity test suggested that the isolates inhibited most pathogenic bacteria by up to 20%,except for A3.4,which inhibited Pasteurella and Staphylococcus aureus by more than 20%.Moreover,the results of the antioxidant test demonstrated that A4 and A3.4 had potent antioxidant activity.In addition,the drug sensitivity test revealed that the isolates were susceptible to commonly used antibiotics.In terms of safety,the isolates promoted growth,enhanced intestinal development,and protected the intestinal barrier without causing any adverse effects.In conclusion,LAB isolated from yak intestinal contents are potential probiotics with excellent antibacterial properties.

关 键 词：LACTOBACILLUS PROBIOTICS IDENTIFICATION Yaks TIBET 

分 类 号：R57[医药卫生—消化系统]