D-阿洛酮糖的高效生物催化合成研究进展  

Research Progress on Efficient Biocatalytic Synthesis of D-Allulose

作　　者：秦慧民 岳世强 叶伟江 王楷喆 高宇昂 QIN Huimin;YUE Shiqiang;YE Weijiang;WANG Kaizhe;GAO Yuang;无(Key Laboratory of Industrial Fermentation Microbiology,Ministry of Education,Tianjin 300457,China;College of Biotechnology,Tianjin University of Science and Technology,Tianjin 300457,China;Shandong Science and Technology Museum,Jinan 250000,China)

机构地区：[1]天津科技大学生物工程学院/工业发酵微生物教育部重点实验室,天津300457 [2]山东省科技馆,山东济南250000

出　　处：《食品科学技术学报》2025年第2期19-28,共10页Journal of Food Science and Technology

基　　金：天津科技大学大学生创新创业训练计划项目(202410057166)。

摘　　要：D-阿洛酮糖是一种新型的具有降血糖、抗氧化等特殊生理功能的低热量稀有糖。目前,D-阿洛酮糖的生物合成主要是利用D-阿洛酮糖3-差向异构酶来催化D-果糖生成D-阿洛酮糖,利用生物酶法大规模合成D-阿洛酮糖的研究重点主要集中在提升差向异构酶的催化活性、热稳定性和耐酸性以满足工业生产的需求。介绍了D-阿洛酮糖的生产现状及其生物酶法合成的最新进展,包括异构酶的分子定向改造、辅助定向进化的超高通量筛选方法、新型酶分子的固定化策略等以提升差向异构酶的工业属性,并进一步探讨了全细胞催化系统在D-阿洛酮糖生产中的应用。研究旨在为D-阿洛酮糖的高效生物催化合成提供理论参考,并提出工业生产中存在的挑战和潜在的解决方案。D-allulose is a novel low-calorie rare sugar with special physiological functions,including blood sugar regulation and antioxidant effects.In present,the biological synthesis of D-allulose mainly utilized D-allulose 3-epimerase to catalyze the conversion of D-fructose into D-allulose.The large-scale biosynthesis of D-allulose using biological enzymes has primarily focused on improving the catalytic activity,thermal stability,and acid tolerance of D-allulose 3-epimerase to meet industrial production requirements.The research progress on the current status of D-allulose production and the latest advances in its biosynthesis,including molecular-directed modification of isomerases,ultra-high-throughput screening assisted by directed evolution,and novel enzyme immobilization strategies to enhance the industrial properties of D-allulose 3-epimerase were summarized.Furthermore,the application of whole-cell catalytic systems in D-allulose production was also discussed.The aim of this review was to provide a reference for the efficient and green biosynthesis of D-allulose and to propose challenges and possible solutions in industrial production.

关 键 词：D-阿洛酮糖 D-阿洛酮糖3-差向异构酶 高通量筛选策略 固定化技术 全细胞催化 

分 类 号：TS201.2[轻工技术与工程—食品科学] TS245.9[轻工技术与工程—食品科学与工程]